摘要
【目的】探明三七地上茎顶部茎段鸡腿状增粗(俗称“拔鸡腿”)对三七传统入药部位氨基酸和三七素含量的影响,为三七优质高产提供理论依据。【方法】采用茚三酮法和高效液相色谱法分别测定三七“非拔鸡腿”和“拔鸡腿”植株主根、侧根、根状茎和地下部器官的总游离氨基酸含量(TFAC)和三七素含量(DC);在此基础上采用方差分析法研究“拔鸡腿”对三七传统入药部位氨基酸和三七素含量的表征效应。【结果】与“非拔鸡腿”相比,2年生“拔鸡腿”主根的TFAC不显著偏低18.92%,根状茎和地下部器官的TFAC分别显著偏低31.06%和16.26%,侧根显著偏高14.26%;主根、侧根和地下部器官的DC分别极显著偏低33.87%、26.55%和17.97%,根状茎极显著偏高9.37%;3年生“拔鸡腿”主根的TFAC显著偏高13.90%,侧根、根状茎和地下部器官分别不显著偏低1.13%、14.32%和1.45%,主根和根状茎分别显著偏低9.96%和8.61%,侧根显著偏高18.14%,地下部器官不显著偏高0.37%;从2年生至3年生,“非拔鸡腿”和“拔鸡腿”侧根、根状茎和地下部器官的TFAC均下降,且后者较前者分别下降673.98%、-31.21%和-44.82%,二者主根的DC均上升,且后者较前者增加5.74倍。【结论】“拔鸡腿”表征着2年生侧根和3年生主根的TFAC较高,2年生根状茎和3年生侧根、地下部器官的DC较高,2年生至3年生时主根TFAC、主根、侧根和地下部器官的DC呈上升趋势;但2年生、3年生其余入药部位的TFAC和DC偏低,且2年生至3年生时各部位的TFAC和DC呈下降趋势。
【Objective】The effects of the top stem segment thickening like chicken leg in the above-ground part of Panax notoginseng(commonly known as“drumstick-forming”)on the amino acid and dencichine contents in the traditional medicinal parts of P.notoginseng were explored to provide the theoretical basis for the good-quality and high-yield of P.notoginseng.【Method】The total free amino acid contents(TFAC)and dencichine content(DC)in the taproots,lateral roots,rhizomes and underground organs of P.notoginseng plants with or without“drumstick-forming”were determined by using ninhydrin method and high performance liquid chromatography,respectively.On this basis,the variance analysis was used to study the characterization effects of“drumstick-forming”on the amino acid and dencichine contents in the traditional medicinal parts of P.notoginseng.【Result】Compared with“non-drumstick-forming”plants,for the 2-year-old“drumstick-forming”ones,TFAC in the taproot lowered with 18.92%at a non-significant level,TFAC in the rhizome and underground organ lowered with 31.06%and 16.26%respectively at significant levels,whereas TFAC in the lateral root increased by 14.26%at a significant level.DC in the taproot,lateral root and underground organ lowered with 33.87%,26.55%and 17.97%respectively at extremely significant levels,whereas DC in the rhizome increased by 9.37%at an extremely significant level.For the 3-year-old“drumstick-forming”ones,TFAC in the taproot increased by 13.90%at a significant level,whereas TFAC in the lateral root,rhizome and underground organ lowered with 1.13%,14.32%and significant levels,whereas DC in the lateral root increased by 18.14%at a significant level and DC in the underground organ increased by 0.37%at a non-significant level.Meanwhile,from 2-to 3-year old,TFAC in the lateral root,rhizome and underground organ of the“non-drumstick-forming”and“drumstick-forming”plants all decreased,and the latter decreased by 673.98%,-31.21%and-44.82%compared to the former,respectivel
作者
周娅
熊芹
魏富刚
赵昶灵
张锐
宫晓云
文国松
ZHOU Ya;XIONG Qin;WEI Fugang;ZHAO Changling;ZHANG Rui;GONG Xiaoyun;WEN Guosong(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming,Yunnan 650201;Wenshan Miaoxiang Sanqi Science and Technology Co.,Ltd.,Wenshan,Yunnan 663000,China)
出处
《贵州农业科学》
CAS
2024年第5期85-93,共9页
Guizhou Agricultural Sciences
基金
国家自然科学基金项目“一年生三七地上茎的黄金分割式紫化及其与皂苷积累的关系”(31460065)
云南农业大学第十五届学生科技创新创业行动基金项目“三七‘拔鸡腿’对其药材三七素含量的表征及其转录水平机制”(2022ZKY237)。
关键词
三七
“拔鸡腿”
入药部位
氨基酸
三七素
表征
Panax notoginseng
“drumstick-forming”
medicinal part
amino acid
dencichine
characterization
