摘要
为了解茶树菇(Agrocybe aegerita)种质资源的遗传多样性和筛选优良的茶树菇新品种,采用菌株拮抗试验方法观察了92株茶树菇菌株间拮抗反应及其类型,ISSR-PCR(inter-simple sequence repeat-PCR)分子标记方法对92株茶树菇菌株的遗传多样性进行了综合分析。拮抗试验将92株茶树菇菌株分为27组;筛选出的20条ISSR引物共扩增出317条清晰条带,多态性条带平均比率为82.60%;在遗传相似系数为0.742时,ISSR分子标记分析可将92株茶树菇划分为6大类群,拮抗试验和ISSR分子标记分析的结果基本一致。通过对比农艺性状分析,初步筛选出滇农5、滇农14、茶5-800、白茶、闽农5及滇农8作为工厂化生产茶树菇菌种。结果表明茶树菇的遗传多样性丰富,结合栽培出菇试验可为茶树菇品种选育和杂交育种的亲本选择提供参考。
To understand the genetic diversity of Agrocybe aegerita germplasm resources and to screen excellent new varieties of Agrocybe aegerit.The antagonistic reaction and its types between strains of Agrocybe aegerita were investi-gated through hyphal antagonism experiment.The genome DNA of A.aegerita was amplified by PCR using inter-simple sequence repeat(ISSR)molecular markers,and the genetic diversity of 92 strains of A.aegerita was analyzed comprehensively.The results showed that the 92 strains were divided into 27 groups based on the antagonistic reac-tion.Among the 20 ISSR primers screened,317 clear bands were amplified,and the average polymorphism band ratio was 82.6%.The results of cluster analysis of ISSR molecular markers showed that at the genetic similarity coefficient level of 0.742,the 92 strains of A.aegerita were divided into six groups.The five strains of Diannong5,Diannong14,Cha5-800,Baicha,Minnong5 and Diannong8 could be used as factory production strains.In this study,the results of antagonism test and ISSR cluster analysis were basically consistent,and the 92 strains selected were rich in genetic diversity.The results of this paper provide a scientific basis for the selection of plant varieties and parental selection of cross breeding for A.aegerita.
作者
王洪秀
章炉军
孙鹏
胡佳
安颖
陈绪涛
魏云辉
WANG Hong-xiu;ZHANG Lu-jun;SUN Peng;HU Jia;AN Ying;CHEN Xu-tao;WEI Yun-hui(Inst.of Agric.App.Microbiol.,Jiangxi Acad.of Agric.Sci.,Nanchang 330200;Inst.of Microbiol.,Jiangxi Prov.Acad.of Sci.,Nanchang 330096;Inst.of Edid.Fungi,Shanghai Acad.of Agric.Sci.,Shanghai 201403;MOE Key Lab.of Biosys.Homeost.&Protect.,Inst.of Biophys.,Coll.of Life Sci.,Zhejiang Uni.,Hangzhou 310058)
出处
《微生物学杂志》
CAS
CSCD
北大核心
2024年第2期19-32,共14页
Journal of Microbiology
基金
国家现代农业产业技术体系建设专项(CARS-20)
国家自然科学基金项目(31460490)
江西现代农业科研协同创新专项(JXXTCXQN201907)
江西现代农业科研协同创新专项(JXXTCX201803-01)
江西省科学院博士基金项目(2022YYB16)
江西省农作物良种联合攻关项目
江西省广昌县科技计划项目(C2023n6)。
关键词
茶树菇
拮抗反应
简单序列重复区间
遗传多样性
农艺性状
tea tree mushroom(Agrocybe aegerita)
antagonistic reaction
ISSR
genetic diversity
agronomic traits
