摘要
[目的 ]探讨朝医方葛根解肌汤对糖尿病小鼠肾损伤的保护作用.[方法 ]取6周龄SPF级雄性小鼠,采用腹腔注射给予链脲佐菌素(150 mg/kg)方式建立糖尿病肾病模型(注射给予链脲佐菌素72 h后检测空腹血糖值≥11.1 mmol/L为制作模型成功),正常组(n=7)腹腔注射给予等体积柠檬水缓冲液.将制作模型成功后的小鼠随机分为模型组、葛根解肌汤低、高剂量组(150、600 mg/kg)和二甲双胍组(0.1 g/kg),每组各7只,灌胃给予相应剂量药物治疗6周.给药期间观察各组小鼠空腹血糖值、体质量及进水量等.于灌胃治疗6周后检测各组小鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、血尿素氮(BUN)及血肌酐(Scr)水平;采用HE染色和PAS染色方法观察各组小鼠肾组织病理形态变化.[结果 ]与模型组比较,葛根解肌汤高剂量组空腹血糖值及饮水量均明显下降(P <0.05),体质量明显升高(P <0.05);血清SOD活性明显升高(P <0.05),MDA、BUN及Scr水平均显著降低(P <0.05);HE染色和PAS染色结果示肾小球损伤程度、细胞排列及增生均有明显好转.[结论]朝医方葛根解肌汤对糖尿病小鼠肾损伤具有保护作用.
OBJECTIVE To investigate the protective effect of Gegen Jieji Decoction on renal injury in diabetic mice.METHODS The 6-week-old SPF male mice were given peritoneal injection of streptozotocin(150 mg/kg)to establish diabetic nephropathy model(fasting blood glucose≥11.1 mmol/L after 72 hours of streptozotocin injection was considered to be successful in making the model),and the normal group(n=7)was given an equal volume of lemonade buffer by peritoneal injection.After the modeling was successful,they were randomly divided into model group,Gegen Jieji Decoction low dose group,high dose group(150,600 mg/kg)and metformin group(0.1 g/kg),7 mice in each group,and were given the corresponding dose of drug by intragastric administration for 6 weeks.Fasting blood glucose,body mass and water intake were observed during administration.The levels of serum superoxide dismutase(SOD),malondialdehyde(MDA),blood urea nitrogen(BUN)and serum creatinine(Scr)were detected after 6 weeks of intragastric treatment.HE staining and PAS staining were used to observe the pathological morphological changes of renal tissue in mice of each group.RESULTS Compared with model group,fasting blood glucose and water intake were significantly decreased(P<0.05),and body mass was significantly increased(P<0.05)in Gegen Jieji Decoction high dose group;serum SOD content was significantly increased(P<0.05),MDA,BUN and Scr levels were significantly decreased(P<0.05);the results of HE staining and PAS staining showed that the degree of glomerular injury,cell arrangement and proliferation were significantly improved.CONCLUSION Gegen Jieji Decoction has protective effect on renal injury of diabetic mice.
作者
韩美慧
任则学
崔荷英
HAN Meihui;REN Zexue;CUI Heying(Yanbian University College of Medicine,Yanji 133002,Jilin,China)
出处
《延边大学医学学报》
CAS
2024年第1期5-8,共4页
Journal of Medical Science Yanbian University
基金
延边大学应用基础研究项目(602023070)。
关键词
糖尿病
葛根解肌汤
肾损伤
小鼠
diabetes
Gegen Jieji Decoction
renal injury
mice
