摘要
目的:探讨胃肠道间质瘤(gastrointestinal stromal tumors,GIST)中黑色素瘤相关抗原基因A12(melanomaassociated antigen gene A12,MAGEA12)的表达水平及其与GIST危险度分级的关系。方法:收集5例GIST患者的肿瘤组织及癌旁组织,采用转录组测序的方法,通过主成分分析(principal components analysis,PCA)、基因集富集分析(gene set enrichment analysis,GSEA)及差异表达基因分析法,明确GIST组织异常信号通路及异常表达基因;采用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)法验证90对GIST组织中异常表达基因MAGEA12的表达水平,并通过χ2检验分析MAGEA12的表达水平与GIST患者临床病理特征的相关性。结果:PCA显示GIST组织基因表达谱与癌旁组织比较差异有统计学意义;GSEA发现,GIST组织中多种肿瘤相关信号通路异常高表达;差异表达基因分析发现,MAGEA12是GIST组织中表达上调最高的基因(表达升高25倍);qRT-PCR验证了MAGEA12在GIST样本中显著高表达,且与GIST危险度分级呈正相关。结论:MAGEA12在GIST中高表达,其表达水平与GIST危险度相关,可能成为治疗的新靶点。
Objective:To investigate the expression level of melanoma-associated antigen gene A12(MAGEA12)in gastrointestinal stromal tumors(GIST)and its relationship with GIST risk classification.Methods:The tumor tissues and adjacent normal tissues of 5 patients with GIST were collected.Principal components analysis(PCA),gene set enrichment analysis(GSEA)and differential expression gene analysis were used to identify the abnormal signaling pathways and abnormal expressed genes in GIST tissues by transcriptome sequencing.Quantitative real-time PCR(qRT-PCR)was used to verify the expression level of MAGEA12 in 90 pairs of GIST tissues.Chi-square test was used to analyze the correlation between the expression level of MAGEA12 and the clinicopathological characteristics of GIST patients.Results:PCA analysis showed that the gene expression profile of GIST tissue was significantly different from that of adjacent cancer control tissue.GSEA analysis found that a variety of tumor-related signaling pathways in GIST tissues were abnormally highly expressed.Differential expression gene analysis showed that MAGEA12 was the gene with the highest upregulation in GIST tissues(25-fold increase in expression).QRT-PCR verified that MAGEA12 was significantly higher expressed in GIST samples and was positively correlated with GIST risk classification.Conclusion:MAGEA12 is highly expressed in GIST,its expression level is correlated with GIST risk,and may become a new target for GIST therapy.
作者
仲铭洋
于卓群
兰东旭
程周扬
吴寒
支小飞
ZHONG Mingyang;YU Zhuoqun;LAN Dongxu;CHENG Zhouyang;WU Han;ZHI Xiaofei(Department of General Surgery,the Affiliated Hospital of Nantong University,Jiangsu 226001)
出处
《南通大学学报（医学版）》
2024年第2期107-111,共5页
Journal of Nantong University(Medical sciences)
基金
国家自然科学基金青年项目(81702369)
中国博士后科学基金面上项目(2016M601868)。
