摘要
为筛选高产胆盐水解酶的乳杆菌,探究其对新生儿黄疸的防治作用。采用添加了25 U/mL制霉菌素的LBS选择性培养基,从健康新生儿粪便和母乳中筛选乳杆菌并鉴定种类;以鼠李糖乳杆菌LGG为阳性对照,体外评估菌株的益生菌特性;利用盐酸苯肼诱导新生SD大鼠黄疸模型,通过分析血清胆红素水平和肝脏组织的损伤情况,以及肝脏炎症因子、核转录因子的相对表达水平,探究高产胆盐水解酶乳杆菌对新生大鼠黄疸的防治作用及机制。结果表明,来自婴儿粪便的格氏乳杆菌FWJL-5在体外具良好的益生特性,并且产胆盐水解酶能力优于LGG,能够显著缓解新生大鼠胆红素水平升高、肝脏组织肿胀和溶血症状,减少肝脏损伤中肝酶的释放,抑制促炎因子的分泌,促进UGt1A1和上游核转录因子孕烷X受体(pregnane X receptor,pXR)、法尼醇X受体(farnesol X receptor,FXR)的表达。综上所述,婴儿粪便来源的格氏乳杆菌FWJL-5可通过上调核受体FXR/pXR促进UGt1A1表达以调节肝脏胆红素代谢,从而减轻新生大鼠黄疸症状,本研究可为格氏乳杆菌防治新生儿黄疸提供新思路。
This study aimed to select Lactobacillus with high bile salt hydrolase(BSH)and to explore its protective role against neonatal jaundice.the LBS selective medium supplemented with 25 U/mL nystatin was used to screen and identify Lactobacillus strains isolated from the feces of healthy newborns and breast milk.L.rhamnosus(LGG)was used as a positive control to evaluate the in vitro probiotic properties and bile salt hydrolase activity of the Lactobacillus strains.thereafter,the protective effect and mechanism of the selected isolates with high bile salt hydrolase activity against phenylhydrazine hydrochloride(pHZ)-induced neonatal jaundice in rats were investigated by analyzing serum bilirubin levels,hepatotoxicity,and the expression of inflammatory cytokines and nuclear transcription factors in the liver.the results showed that compared with the LGG,L.gasseri FWJL-5,which was isolated from infant feces,exhibited outstanding in vitro probiotic properties and higher bile salt hydrolase activity than LGG.L.gasseri FWJL-5 significantly alleviated the increase in serum bilirubin levels,liver tissue swelling and hemolysis in pHZ-treated neonatal rats,reduced the release of hepatic enzymes,inhibited the secretion of proinflammatory cytokines,and promoted the expression of UGt1A1,pregnane X receptor(pXR),and farnesol X receptor(FXR).taken together,L.gasseri FWJL-5 could regulate bilirubin metabolism in the liver by up-regulating the expression of FXR/pXR and UGt1A1,thereby alleviating neonatal jaundice in rats.Our findings may provide new ideas for the prevention and treatment of neonatal jaundice by L.gasseri.
作者
张慧敏
李彬彬
潘晓花
孙嘉
ZHANG Huimin;LI Binbin;PAN Xiaohua;SUN Jia(School of Food Science and technology,Jiangnan University,Wuxi 214122,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第11期118-127,共10页
Food Science
基金
江苏省杰出青年基金资助项目(BK20200026)。
关键词
格氏乳杆菌
胆盐水解酶
新生儿黄疸
胆红素代谢
Lactobacillus gasseri
bile salt hydrolase
neonatal jaundice
bilirubin metabolism
