摘要
目的分析HIV-1抗体筛查有反应性样本的再复检和补充试验(核酸试验和抗体确证试验)检测结果,为HIV检测策略提供依据。方法2022年2月—2023年2月河南省某市艾滋病确证实验室对医疗机构送检的141份HIV-1抗体筛查有反应性样本进行抗体再复检、病毒载量(VL)检测,并采用免疫印迹法(WB)进行抗体确证试验。结果141份HIV-1抗体筛查有反应性样本再复检,有反应131份(92.91%),无反应10份(7.09%)。按人群分类再复检阳性率由低到高依次为孕产妇50.00%(1/2)、术前检测91.67%(77/84)、自愿咨询94.74%(36/38)、体检(婚检、招兵体检和正常体检)100.00%(5/5)、羁押人员100.00%(7/7)。对131份再复检有反应样本进行HIV核酸检测,其中13份(9.92%)检测值<50拷贝/mL,8份(6.11%)为50~<1000拷贝/mL,13份(9.92%)为1000~<5000拷贝/mL,97份(74.05%)检测值≥5000拷贝/mL。对21份VL检测值在50~<5000拷贝/m L的样本进行免疫印迹法检测,结果均为HIV-1抗体阳性,其中出现9种带型,以全条带为主。再复检无反应的10份样本均未出现HIV抗体特异性条带,VL检测结果均<50拷贝/mL。结论采用再复检和病毒载量检测等多种方法,降低HIV-1病毒载量阳性阈值,可提高HIV阳性检出率,快速锁定传染源。
Objective To analyze the second retest and complementary tests(nucleic acid test and antibody confirmation test)results of HIV-1 antibody screening reactive samples,and provide the evidence for HIV-1 detection strategy.Methods From February 2022 to February 2023,141 HIV-1 antibody screening reactive samples sent by medical institutions were tested for antibody second retest,viral load(VL),and antibody confirmation test by Western blot(WB).Results Among the 141 HIV-1antibody screening reactive samples,131(92.91%)samples had response,and 10(7.09%)samples had no response in the second retest.The positive rate in the second retest was 50%(1/2)for pregnant women,91.67%(77/84)for preoperative examination,94.74%(36/38)for voluntary consultation,100.00%(5/5)for physical examination(marriage examination,recruitment examination and normal physical examination),and 100.00%(7/7)for other persons(detainees and previous confirmed patients).HIV viral load detection results were<50 copies/mL for 13(9.92%)samples,between 50-<1000 copies/mL for 8(6.11%)samples,between 1000-5000 copies/mL for 13(9.92%)samples,and≥5000 copies/mL for 97(74.05%)samples.Of the 21samples with VL detection results between 50-5000 copies/mL,WB results were all HIV-1 antibody positive,and 9 band types were found.They were mainly full band.There were no bands in 10 samples of no response after the second retest,and VL detection results were all<50 copies/mL.Conclusions It can improve the level of HIV detection rate and quickly lock the source of infection to reduce the HIV-1 viral load positive threshold and use antibody retest and nucleic acid detection.
作者
闫江舟
李绍岗
刘家琪
任高翔
任亭亭
张向兵
耿杰
张国龙
YAN Jiangzhou;LI Shaogang;LIU Jiaqi;REN Gaoxiang;REN Tingting;ZHANG Xiangbing;GENG Jie;ZHANG Guolong(Henan Center for Disease Control and Prevention,Zhengzhou,Henan 450016,China;Jiaozuo Center for Disease Control and Prevention)
出处
《现代疾病预防控制》
2024年第4期261-264,共4页
MODERN DISEASE CONTROL AND PREVENTION
基金
国家“十三五”科技重大专项(2018ZX10715009)
河南医学科技攻关计划项目(LHGJ20220148)。
