摘要
目的:通过高通量测序技术检测白鲜皮-苦参致Wistar大鼠肝损伤后的微生物变化,探讨对白鲜皮-苦参致肝损伤的潜在微生物机制。方法:雌性Wistar大鼠随机分为正常组、中药低、中、高剂量组4组,以白鲜皮-苦参不同剂量(生药量分别为4.125、8.25、16.5 g·kg^(-1))灌胃28 d,记录一般情况,计算肝脏体质量比、观测血清生化指标、苏木素-伊红(HE)染色观察肝脏病理学改变、16S rDNA高通量测序检测大鼠粪便微生物变化。结果:与正常组比较,白鲜皮-苦参给药后Wistar大鼠肝脏重量和肝脏体质量比增加,中药中、高剂量组肝脏重量差异有统计学意义(P<0.05),中药低、中、高剂量组肝脏体质量比均具有统计学意义(P<0.05)。与正常组比较,中药中、高剂量组血清白蛋白、胆固醇水平增加(P<0.05)。中药中、高剂量组肝脏组织病理学出现微小空泡样改变。与正常组比较,白鲜皮-苦参给药后出现明显的肠道菌群紊乱,中药中、高剂量组α多样性增加。主坐标分析显示,随着给药剂量的增加,物种越来越偏离正常组。与正常组比较,给药后厚壁菌门/拟杆菌门比例下降,属水平的Parasutterella、 Romboutsia、 Turicibacter、 Allobaculum、 Dubosiella升高,Lachnospiraceae_NK4A136_group、 Blautia、Erysipelatoclostridium、Muribaculum、Ruminococcus_gnavus_group降低。相关性分析表明,Parasutterella、Romboutsia、Turicibacter、Allobaculum、Dubosiella与血清胆固醇、肝脏体质量比正相关,lanchnospiraceae_NK4A136_group、Blautia、Muribaculum、Erysipelatoclostridium、Ruminococcus_gnavus_group与血清胆固醇、肝脏体质量比负相关。结论:白鲜皮-苦参的肝损伤表现为脂代谢紊乱,其机制与脂代谢相关微生物增加有关。
Objective: To observe the microbial changes in Wistar rats with liver injury caused by the Dictamni Cortex-Flavescens Sophora by high-throughput sequencing technology and investigate the potential mechanism of liver injury caused by the Dictamni Cortex-Flavescens Sophora. Method: Female Wistar rats were randomly divided into four groups: normal group, as well as low-dose, medium-dose, and high-dose groups of traditional Chinese medicine(TCM). The rats were gavaged with the Dictamni Cortex-Flavescens Sophora in different doses(4.125, 8.25, 16.5 g·kg^(-1) of raw drug respectively) for 28 days, and the general condition was recorded. The liver-body weight ratio was calculated, and the biochemical indexes of serum were observed. The Hematoxylin-eosin(HE) staining was used to observe pathological changes in the liver, and 16S rDNA high-throughput sequencing was utilized to detect fecal microbial changes in rats. Result: Compared with the normal group, Dictamni Cortex-Flavescens Sophora increased the liver weights and liver-body weight ratios of Wistar rats. The difference in liver weight between the medium-dose and high-dose groups of TCM was statistically significant(P<0.05), and the liver-body weight ratios of the low-dose, medium-dose, and highdose groups of TCM were all statistically significant(P<0.05). Compared with the normal group, serum albumin and cholesterol levels increased in the medium-dose and high-dose groups of TCM(P<0.05). The histopathology of the liver in the medium-dose and high-dose groups of TCM showed tiny vacuole-like changes.Compared with the normal group, there were obvious intestinal flora disorders after administration of Dictamni Cortex-Flavescens Sophora, and alpha diversity increased in the medium-dose and high-dose groups of TCM.The principal coordinates analysis showed that species increasingly deviated from the normal group as the administered dose increased. Compared with the normal group, the proportion of Firmicutes and Bacteroidota decreased after the drug administration, and th
作者
牛露娜
王春国
荆鲁
NIU Luna;WANG Chunguo;JING Lu(Eye Hospital,China Academy of Chinese Medical Sciences,Beijing 100040,China;Beijing Institute of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2024年第11期34-42,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81274181)。
关键词
脂代谢紊乱
肠道微生物
16S
rDNA测序
相关性分析
中药肝损伤
白鲜皮-苦参
lipid metabolism disorder
intestinal microorganisms
16S rDNA sequencing
correlation analysis
liver injury caused by traditional Chinese medicine(TCM)
Dictamni Cortex-Flavescens Sophora
