摘要
目的了解临沂市手足口病病原情况,并对柯萨奇病毒A10型(Coxsackievirus A10,CV-A10)完整VP1区基因进行基因特征分析。方法对临沂市2023年手足口病例样本进行检测分型及毒株分离,扩增CV-A10分离株VP1区基因并进行测序,将获得的序列与NCBI数据库中的序列进行比对,构建系统进化树,进行基因特征及分子流行病学分析。结果2023年全年共采集手足口病样本861例,核酸检测阳性594例(68.99%),阳性病例中男女比例1.56∶1。5岁以内儿童占81.65%,高发季节为6~8月份(83.84%)。病原体以CV-A6为主(84.51%),其次为CV-A10(9.93%)。13株CV-A10分离株株间VP1区基因序列核苷酸和氨基酸同源性分别为93.29%~100.00%、97.65%~100.00%,与A型原型株AF081300-Kowalik/USA/1950的核苷酸和氨基酸同源性较低(75.95%~76.62%、91.72%~92.41%)。与C2c代表株的氨基酸和核苷酸同源性最高(94.28%~96.76%、98.28%~100.00%),遗传距离最近(0.04~0.06)。氨基酸位点变异分析显示,分离株与原型株AF081300-Kowalik/USA/1950相比存在较多位点变异,与C2c代表株相比仅部分分离株发生I80V、E141K、P147S、T219I、E240K、V261I位点突变。遗传进化树显示分离株均与C2c代表株在同一分支,均属C2c基因型,且分离株进一步分为2个较小分支。结论2023年临沂市手足口病病原以CV-A6为主,其次为CV-A10。CV-A10分离株均为C2c基因型,并可分为2个进化分支,应加强对CV-A10的持续监测和基因特征分析。
Objective To understand the pathogenic situation of hand,foot and mouth disease(HFMD)in Linyi City,and to analyze the gene characteristics of Coxsackievirus A10 complete VP1 region.Methods The samples of HFMD cases from Linyi City in 2023 were tested for typing and strain isolation,and the VP1 gene of CV-A10 isolate was amplified and sequenced.The obtained sequences were compared with those in the NCBI database,and the phylogenetic tree was constructed for gene characteristics and molecular epidemiological analysis.Results In 2023,a total of 861 samples of HFMD were collected,and 594(68.99%)were positive for nucleic acid tests.The male to female ratio of positive cases was 1.56∶1.Children under 5 years old accounted for 81.65%,and the highest incidence season was from June to August(83.84%).CV-A6 was the main pathogen(84.51%),followed by CV-A10(9.93%).The nucleotide and amino acid homology of VP1 gene sequence among 13 isolates were 93.29%-100.00%and 97.65%-100.00%,respectively.The nucleotide and amino acid homology with AF081300-Kowalik/USA/1950 was lower(75.95%-76.62%,91.72%-92.41%).The amino acid and nucleotide homology with C2c was the highest(94.28%-96.76%,98.28%-100.00%),and the genetic distance was the closest(0.04-0.06).Amino acid site variation analysis showed that compared with the prototype strain AF081300-Kowalik/USA/1950,the isolates had more site variation,while only some isolates had I80V,E141K,P147S,T219I,E240K and V261I mutations compared with the representative strain C2c.The genetic evolution tree showed that the isolates were all in the same clade as the C2c representative strains,and they all belonged to the C2c genotype,and the isolates were further divided into two smaller clades.Conclusions In 2023,CV-A6 was the main pathogen of HFMD in Linyi City,followed by CV-A10.All CV-A10 isolates were C2c genotypes and can be divided into two evolutionary clades.Continuous monitoring and genetic characterization of CV-A10 should be strengthened.
作者
蒋春云
季圣翔
王传宝
刘祥亮
尹德清
张戈
Jiang Chunyun;Ji Shengxiang;Wang Chuanbao;Liu Xiangliang;Yin Deqing;Zhang Ge(Linyi Center for Disease Control and Prevention,Linyi 276001,China;The Second Affiliated Hospital of Shandong First Medical University,Taian 271000,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2024年第2期150-155,共6页
Chinese Journal of Experimental and Clinical Virology
