摘要
目的:通过检测肝郁化火型迟发性痤疮女性病人血清趋化因子CCL5、CXCL10表达水平,分析其与病人病情关系。方法:收集80例肝郁化火型迟发性痤疮女性病人作为痤疮组,根据痤疮Pillsbury国际改良分级法分为轻组(n=23)、中组(n=32)、重组(n=25)。以同时段一般资料有可比性的80名女性体检健康者作为对照组。采用酶联免疫吸附法检测血清CCL5、CXCL10表达水平以及炎性因子白细胞介素(IL)-1β、干扰素-γ(IFN-γ)、IL-2水平,并分析其在各组中表达差异。Pearson法分析CCL5、CXCL10与IL-1β、IFN-γ、IL-2的相关性。结果:痤疮组血清CCL5、CXCL10、IL-1β、IFN-γ、IL-2表达水平均高于对照组,差异有统计学意义(P<0.01)。血清CCL5、CXCL10表达水平与痤疮组病人年龄、体质量指数、经期状态无关(P>0.05);月经前加重病人血清CCL5、CXCL10表达水平高于月经前无加重者,差异有统计学意义(P<0.01)。重组、中组及轻组血清CCL5、CXCL10、IL-1β、IFN-γ、IL-2表达水平逐次降低,差异有统计学意义(P<0.05)。相关性显示,CCL5、CXCL10均与IL-1β、IFN-γ、IL-2存在正相关关系(P<0.05~P<0.01)。结论:肝郁化火型迟发性痤疮女性病人血清中CCL5、CXCL10呈上调表达,病情越重,其表达水平越高,可为临床评估和治疗迟发性痤疮病情严重度提供参考。
Objective:To detect the expression levels of serum chemokines CCL5 and CXCL10 in female patients with delayed acne of stagnation of liver fire,and analyze the relationship between them and the patient′s condition.Methods:A total of 80 female patients with delayed acne of stagnation of liver fire were collected as the acne group.According to the Pillsbury international modified classification method of acne,they were divided into mild group(n=23),moderate group(n=32)and severe group(n=25).A total of 80 female healthy subjects with comparable general information during the same period were selected as the control group.The expression levels of serum CCL5 and CXCL10 and the levels of inflammatory factors interleukin(IL)-1β,interferon-γ(IFN-γ)and IL-2 were detected by enzyme-linked immunosorbent assay,and the differences in each group were analyzed.The correlation of CCL5 and CXCL10 with IL-1β,IFN-γand IL-2 was analyzed by Pearson method.Results:The expression levels of serum CCL5,CXCL10,IL-1β,IFN-γand IL-2 in the acne group were higher than those in the control group,and the difference was statistically significant(P<0.01).The expression levels of serum CCL5 and CXCL10 were not related to the age,body mass index and menstrual status of the acne group(P>0.05).The expression levels of serum CCL5 and CXCL10 in patients with premenstrual aggravation were higher than those without premenstrual aggravation,and the difference was statistically significant(P<0.01).The expression levels of serum CCL5,CXCL10,IL-1β,IFN-γ,and IL-2 in the severe,moderate and mild groups were gradually decreased,and the difference was statistically significant(P<0.05).The correlation analysis showed that CCL5 and CXCL10 were positively correlated with IL-1β,IFN-γand IL-2(P<0.05 to P<0.01).Conclusions:The expression of CCL5 and CXCL10 is up-regulated in the female patients with delayed acne of stagnation of liver fire.The more severe the disease,the higher the expression level,which can provide a reference for clinical evaluation and
作者
李婧
曹洋
LI Jing;CAO Yang(Department of Dermatology,Tangshan Hospital of Traditional Chinese Medicine,Tangshan Hebei 063000,China;Department of Rehabilitation,Tangshan Hospital of Traditional Chinese Medicine,Tangshan Hebei 063000,China)
出处
《蚌埠医学院学报》
CAS
2024年第4期501-505,共5页
Journal of Bengbu Medical College
基金
河北省中医药管理局科研计划项目(2020382)。