摘要
铝是酸性土壤中导致植物生长受阻的关键因素,为阐明柠檬酸对铝胁迫下菊芋叶片的生理响应,以耐铝性差异较大的南京菊芋(NJ H.t)和资阳菊芋(ZY H.t)为试验材料,对其进行不同浓度(0、350、700μmol·L^(-1))铝胁迫,同时分别施加0、30、60、90μmol·L^(-1)柠檬酸(Citric Acid,CA),探究对菊芋叶片各时期(10、20、30 d)叶面积、抗氧化系统、光化学系统、营养元素含量及遗传系统稳定性的影响,并对其耐铝机理进行探究.结果表明:铝胁迫抑制了两种菊芋光合电子传递效率和PSⅡ活性,并对抗氧化系统产生损伤,且随浓度升高抑制程度加剧;多组数据显示CA浓度为60μmol·L^(-1)时,对铝胁迫缓解效果最佳,但两种菊芋生理变化峰值时间存在差异.施加CA后叶面积抑制现象减弱,SOD、POD、CAT活性均显著增加;叶绿素a、b含量及F_(v)/F_(m)有较大增幅的同时,初始荧光F_(0)及G_(s)、P_(n)和T_(r)均有所下降,光合转化效率增强.同时,施加柠檬酸能够促进菊芋对N、Ca、Mg、P的吸收,并抑制K元素的吸收,其中,柠檬酸对菊芋叶片Ca含量作用最为明显.此外,柠檬酸可使细胞核拖尾现象得到抑制,菊芋DNA损伤有所缓解.本研究通过各指标测定,初步了解铝胁迫下菊芋在生理上的耐铝机制,为酸性土壤下的菊芋种植提供理论依据.
Aluminum is a key factor in stunted plant growth in acidic soils.To explore the physiological regulatory effect of citric acid on Helianthus tuberosus leaves under aluminum stress,Nanjing H.tuberosus(NJ H.t) and Ziyang H.tuberosus(ZY H.t),which with enormous differences in aluminum tolerance,were used as test materials.Antioxidant system,photochemical system,nutrient content,and genetic system stability of H.tuberosus leaves at various stages(10,20 and 30 d) were explored with 0,350,700 μmol·L^(-1) aluminum treatment while applying 0,30,60,90 μmol·L^(-1)citric acid on leaf area and its mechanism of aluminum tolerance was also explored.The results showed that aluminum stress inhibited the photosynthetic electron transfer efficiency and PS Ⅱ activity of H.tuberosus under both treatments,damaged the antioxidant system,which was inhibited with the increase of concentration.Several groups of data showed that the 60 μmol·L^(-1) citric acid treatment had the best alleviating effect on alleviating aluminum stress,but the peak times of physiological changes of two H.tuberosus cultivars were different.After CA application,the inhibition of leaf area was weakened,and the activities of SOD,POD and CAT,also the F_v/F_(m) were significantly increased under the co-treatment of aluminum and citric acid.The initial fluorescence F_0,and G_s,P_(n) and T_(r) decreased significantly,and photosynthetic conversion efficiency increased.At the same time,the application of citric acid can promote the absorption of N,Ca,Mg,and P elements,and inhibit the absorption of K elements in H.tuberosus.Among them,citric acid had the most obvious effect on the Ca content of H.tuberosus leaves.In addition,citric acid can inhibit the tailing phenomenon of the nucleus,and the DNA damage of H.tuberosus is alleviated.In this study,the physiological aluminum tolerance mechanism of H.tuberosus under aluminum stress was preliminarily understood by measuring various methods,which provided theoretical basis for H.tuberosus planting in acidic soil.
作者
鲍若妍
俞奕汝
王娴
李春俣
孙靖菲
庄家莹
邵甜甜
吴文静
叶梦瑶
赵杭
沈璐垚
刘鹏
洪华嫦
BAO Ruoyan;YU Yiru;WANG Xian;LI Chunyu;SUN Jingfei;ZHUANG Jiaying;SHAO Tiantian;WU Wenjing;YE Mengyao;ZHAO Hang;SHEN Luyao;LIU Peng;HONG Huachang(College of Life Sciences,Zhejiang Normal University,Jinhua 321004;College of Geography and Environmental Sciences,Zhejiang Normal University,Jinhua 321004)
出处
《环境科学学报》
CAS
CSCD
北大核心
2024年第4期368-380,共13页
Acta Scientiae Circumstantiae
基金
国家自然科学基金(No.32001224,41702181)
2022年国家级大学生创新创业训练计划(No.202210345052)。
关键词
菊芋
铝胁迫
柠檬酸
叶面积
抗氧化系统
光化学系统
营养元素
DNA损伤
Helianthus tuberosus L.
aluminum stress
citric acid
leaf area
antioxidant system
photosynthetic systems
nutrient elements
DNA damage
