摘要
为明确河北省深泽县北苍术种植田根蛆为害情况、根蛆种类及苏云金芽胞杆菌对根蛆的田间防治效果,采用5点取样法调查北苍术根蛆的为害情况,并利用mtCOI分子标记和成虫形态对比鉴定其种类,同时施用苏云金芽胞杆菌JQD117水剂对北苍术根蛆进行田间防治。结果表明,深泽县北苍术根蛆为害率为2.0%~25.0%,死苗率高达10%,单株平均虫口数量可达27.4头。北苍术根蛆mtCOI基因序列与韭菜迟眼蕈蚊的一致性为100%,其成虫形态特征与韭菜迟眼蕈蚊形态特征一致,确定北苍术根蛆为韭菜迟眼蕈蚊。苏云金芽胞杆菌JQD117水剂8000m L/亩对北苍术根蛆的防治效果和保株效果分别为70.72%和79.21%。本研究明确了为害深泽县北苍术的根蛆种类、为害情况及苏云金芽胞杆菌JQD117的田间防治效果,旨在为今后北苍术根蛆的绿色防控提供科学依据。
Damage of root maggots in the fields of Atractulodes chinensis in Shenze Country,Hebei Province were investigated using a five-point sampling method and the species of root maggots were identified by mitochondrial cytochrome oxidase I(mtCOI)molecular marker and morphological characteristics of the adult.At the same time,Bacillus thuringiensis JQD117 aqueous solution was tested for control of the root maggots in A.chinensis field.The results showed that the damage rate of A.chinensis by root maggots in Shenze Country was 2.0%—25.0%,the death rate was as high as 10%,and the average number of insects per plant was 27.4.The mtCOI gene sequences of A.chinensis root maggots were 100%consistent with that of Bradysia odoriphaga,and the morphological characteristics of A.chinensis root maggot’s adult were basically the same as those of B.odoriphaga,which indicates that A.chinensis root maggots are B.odoriphaga.The control of B.thuringiensis JQD117 aqueous solution 8000 mL/mu against the A.chinensis root maggots in the field was 70.72%and the undamaged plants accounted for 79.21%.This study has clarified the species and damage of root maggots in A.chinensis in Shenze Country,and the field control efficacy of B.thuringiensis JQD117,which is of significance for the green control of the root maggots of A.chinensis in the future.
作者
程佳旭
丰硕
郝芯仪
苏亚
曹伟平
李耀发
贾海民
宋健
CHENG Jiaxu;FENG Shuo;HAO Xinyi;SU Ya;CAO Weiping;LI Yaofa;JIA Haimin;SONG Jian(Institute of Plant Protection,Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture/IPM Center of Hebei Privince/International Science and Technology Joint Research Center on IPM of Hebei Province,Baoding 071000,China;Agriculture,Rural and Water Resources Bureau of Boye County,Boye 071300,China)
出处
《中国生物防治学报》
CSCD
北大核心
2024年第2期491-496,共6页
Chinese Journal of Biological Control
基金
国家特色蔬菜产业体系(CARS-24-C-02)
河北省农林科学院现代农业科技创新专项课题(2022KJCXZX-ZBS-6)
河北省重点研发项目(21326505D)。
关键词
北苍术
韭菜迟眼蕈蚊
种类鉴定
分子标记
苏云金芽胞杆菌
田间防治
Atractulodes chinensis
Bradysia odoriphaga
species identification
molecular marker
Bacillus thuringiensis
field control
