摘要
目的探究影响气相色谱-串联质谱法测定人参中33种农药残留量时测量不确定度的主要因素。方法采用气相色谱-串联质谱法对人参中33种农药残留量进行测定,根据JJF 1059.1-2012《测量不确定度评定与表示》和JJF 1135-2005《化学分析测量不确定度评定》要求,建立不确定度评定的数学模型,分析在测定过程中的主要不确定度来源,即从样品的称量、标准溶液的稀释、样品的前处理和仪器测定的重复性等方面对各个分量进行评估。结果测量不确定度主要来源于回收率实验,样品平均回收率为79.61%~116.70%,33种农药残留在0.010~0.20 mg·kg^(-1)范围测量结果的扩展不确定度为±0.000718~±0.0252 mg·kg^(-1)。结论本评定方法为气相色谱-串联质谱法同时测定人参中33种农药残留量在临界点附近的结果判定提供了理论依据。
Objective To explore the main factors of uncertainty in the determination of 33 pesticide residues in ginseng by gas chromatography-tandem mass spectrometry.Methods 33 pesticide residues in ginseng were determined by gas chromatography-tandem mass spectrometry.According to the requirements of JJF 1059.1—2012"Evaluation and Expression of Uncertainty in Measurement"and JJF 1135—2005"Evaluation of Uncertainty in Chemical Analysis Measurement",the mathematical model of uncertainty evaluation was established.Each component is evaluated by analyzing the main sources of uncertainty in the determination process,namely the weighing of the samples,the dilution of the standard solution,the pretreatment of the samples,and the repeatability of the instrumental determination.Results The average recovery rate of samples ranged from 79.61%to 116.70%.The extended uncertainty of 33 pesticide residues measured in the range of 0.010 to 0.20 mg·kg^(-1) was±0.000718 to±0.0252 mg·kg^(-1).Conclusion This method provides a theoretical basis for the determination of 33 pesticide residues in ginseng by gas chromatography-tandem mass spectrometry near the critical point.
作者
宋莹莹
李健豪
郭畅冰
谢丽娟
王国明
徐芳菲
SONG Yingying;LI Jianhao;GUO Changbing;XIE Lijuan;WANG Guoming;XU Fangfei(Plant Chemistry Laboratory,Jilin Ginseng Research Institute,Changchun 130031,China)
出处
《数理医药学杂志》
CAS
2024年第5期323-333,共11页
Journal of Mathematical Medicine
关键词
气相色谱-串联质谱法
人参
农药残留量
同时测定
不确定度评定
Gas chromatography-tandem mass spectrometry
Ginseng
Pesticide residue
Simultaneous determination
Evalution of uncertainty
