摘要
目的 探讨老年重症肺炎患者血清叉头状转录因子1(FOXO1)、自噬相关基因7(ATG7)的表达水平及与短期预后的关系。方法 选取2020年8月至2022年8月该院收治的122例老年重症肺炎患者作为病例组,另选取同期来该院进行健康体检的105例老年人作为对照组。采用酶联免疫吸附试验(ELISA)检测血清FOXO1、ATG7的表达水平。根据患者重症肺炎确诊后28 d内是否死亡分为存活组(n=91)和死亡组(n=31)。采用受试者工作特征(ROC)曲线评估血清FOXO1、ATG7表达水平对老年重症肺炎短期死亡的预测价值,采用多因素Logistic回归分析探讨老年重症肺炎短期死亡的影响因素。结果 病例组患者血清FOXO1、ATG7表达水平明显高于对照组,差异有统计学意义(t=7.615、29.591,P<0.05);老年重症肺炎死亡组患者血清FOXO1、ATG7表达水平均高于生存组,差异有统计学意义(t=10.029、9.399,P<0.05)。血清FOXO1、ATG7预测老年重症肺炎患者短期死亡的曲线下面积(AUC)分别为0.733(95%CI:0.673~0.799)、0.826(95%CI:0.756~0.893),两者联合预测的AUC为0.908(95%CI:0.862~0.949)。FOXO1、ATG7与急性生理与慢性健康评估(APACHEⅡ)评分呈正相关(r=0.693、0.627,均P<0.05)。多因素Logistic回归分析显示,APACHEⅡ评分≥22.56分(OR=3.589,95%CI:1.714~7.515),FOXO1≥10.67 ng/mL(OR=2.529,95%CI:1.232~5.193),ATG7≥16.35 pg/mL(OR=2.875,95%CI:1.414~5.845)是老年重症肺炎患者死亡的危险因素(P<0.05)。结论 老年重症肺炎患者血清FOXO1、ATG7表达水平明显升高,二者可用于评估老年重症肺炎患者的短期预后。
Objective To explore the levels of serum forkhead transcription factor 1(FOXO1)and autophagy-related gene 7(ATG7)in elderly patients with severe pneumonia and their relationship with short-term prognosis.Methods A total of 122 elderly patients with severe pneumonia admitted to the hospital from August 2020 to August 2022 were selected as the case group,and 105 elderly people who came to the hospital for physical examination during the same period were selected as the control group.The serum levels of FOXO1 and ATG7 were detected by enzyme-linked immunosorbent assay(ELISA).The patients were divided into the survival group(n=91)and the death group(n=31)according to whether they died within 28 days after the diagnosis of severe pneumonia.Receiver operating characteristic(ROC)curve was used to evaluate the predictive value of serum FOXO1 and ATG7 levels for short-term mortality in elderly patients with severe pneumonia.The influencing factors for short-term mortality in elderly patients with severe pneumonia were explored by multivariate Logistic regression analysis.Results The serum FOXO1 and ATG7 levels in the case group were higher than those in the control group,and the differences were statistically significant(t=7.615,29.591,P<0.05).The serum expression levels of FOXO1 and ATG7 in the death group were higher than those in the survival group,and the differences were statistically significant(t=10.029,9.399,P<0.05).The area under the curve(AUC)of serum FOXO1 and ATG7 for predicting short-term mortality in elderly patients with severe pneumonia was 0.733(95%CI:0.673-0.799)and 0.826(95%CI:0.756-0.893),respectively,and the AUC of FOXO1 combined with ATG7 for predicting short-term mortality in elderly patients with severe pneumonia was 0.908(95%CI:0.862-0.949).FOXO1 and ATG7 were positively correlated with acute physiology chronic health evaluation(APACHEⅡ)score(r=0.693,0.627,P<0.05).The results of multivariate Logistic regression showed that APACHEⅡscore≥22.56(OR=3.589,95%CI:1.714-7.515),FOXO1≥10.67 ng/mL(OR
作者
生淑红
许靖
付佑辉
SHENG Shuhong;XU Jing;FU Youhui(Department of Respiratory Medicine,Shanghai Seventh People′s Hospital,Shanghai 200137,China)
出处
《国际检验医学杂志》
CAS
2024年第10期1218-1222,共5页
International Journal of Laboratory Medicine
基金
浦东新区科技发展基金项目(PKJ2020-Y14)。
关键词
重症肺炎
叉头状转录因子1
自噬相关基因7
预后
老年
severe pneumonia
forkhead transcription factor 1
autophagy related gene 7
prognosis
elderly
