摘要
目的:利用高效液相色谱-串联三重四极杆质谱(HPLC-MS/MS)对保健食品中6种大麻素进行定性定量分析。方法:HLB固相萃取小柱净化,采用XBridge C18色谱柱,乙腈溶液作为有机相,10mmol·L^(-1)乙酸铵水溶液作为水相,2种离子模式同时扫描,同位素内标定量。结果:所测大麻素在1-100μg·L^(-1)浓度范围均呈现良好线性关系,相关系数(r^(2))均>0.999。方法的检出限和定量限分别为5μg·kg^(-1)和10μg·kg^(-1)。该方法稳定性好,灵敏度高,适用于常见保健食品中6种大麻素的定性定量分析。结论:本研究成功建立了保健食品中6种大麻素的定性定量分析,为保健食品品质评价、安全监管及风险防范提供技术支持。
Objective:Qualitative and quantitative analysis of six cannabinoids in health food using high-performance liquid chromatography tandem triple quadrupole mass spectrometry(HPLC-MS/MS).Methods:HLB solid phase extraction column purification,using XBridge C18 chromatography column,with acetonitrile solution as the organic phase and 10 mmol·L^(-1)ammonium acetate aqueous solution as the aqueous phase.Two ions modes were scanned simultaneously and isotope internal standard quantification was performed.Results:The measured Cannabinoids between 1-100μg·L^(-1)concentration range shows a good linear relationship,and the correlation coefficients(r^(2))is greater than 0.999.The detection limit and quantification limit of the method are 5μg·kg^(-1)and 10μg·kg^(-1).The method has good stability and high sensitivity,and is suitable for qualitative and quantitative analysis of six Cannabinoid in common health products.Conclusion:This study successfully established established a qualitative and quantitative analysis of six cannabinoids in health food,providing technical support for quality evaluation,safety supervision,and risk prevention of health food.
作者
侯巧红
张勋
赵宇
齐鹤鸣
李孟璇
步献功
董昕
李燕鹭
宋清莲
邹明强
金明华
胡婷婷
Hou Qiaohong;Zhang Xun;Zhao Yu;Qi Heming;Li Mengxuan;Bu Xiangong;Dong Xin;Li Yanlu;Song Qinglian;Zhou Mingqiang;Jin Minghua;Hu Tingting(Jilin University,School of Public Health,Changchun 130021,China;Changchun Customs Technology Center,Changchun 130062,China;Science and Technology Research Center of China Customs,Beijing 100026,China;Jilin Provincial Institute of Sports Science(Jilin Provincial Anti-Doping Center),Changchun 130000,China;China Academy of inspection and quarantine Sciences,Beijing 100176,China)
出处
《现代科学仪器》
2024年第2期10-15,共6页
Modern Scientific Instruments
基金
海关总署科研项目2020HK182
海南省重点研发计划项目ZDYF2021SHFZ069
吉林省科技发展计划项目(重点研发项目)20210203045SF
中国海关科学技术研究中心研课题2023HX07。
关键词
大麻素
保健食品
高效液相色谱-串联质谱
Cannabinoids
Health food
High performance liquid chromatography-tandem mass spectrometry
