摘要
目的 研究川芎嗪调节Yes相关蛋白(YAP)/转录辅激活因子(TAZ)信号通路对宫颈癌细胞上皮间质转化和放疗耐药性的影响。方法 将人宫颈癌细胞株C33A随机分为对照组(不做任何干预处理)、川芎嗪组(1.5 mg·mL^(-1)川芎嗪)、川芎嗪+空载组(1.5 mg·mL^(-1)川芎嗪+转染空载质粒)、川芎嗪+YAP1过表达组(1.5 mg·mL^(-1)川芎嗪+转染YAP1过表达质粒)。构建人宫颈癌放射线抵抗细胞C33A-RR并随机分为放射抵抗对照组(不做任何干预处理)、放射组(6 Gy射线)、放射+川芎嗪组(6 Gy射线+1.5 mg·mL^(-1)川芎嗪)、放射+川芎嗪+空载组(6 Gy射线+1.5 mg·mL^(-1)川芎嗪+转染空载质粒)、放射+川芎嗪+YAP1过表达组(6 Gy射线+1.5 mg·mL^(-1)川芎嗪+转染YAP1过表达质粒)。用蛋白质印迹法检测蛋白相对表达水平;用细胞划痕和Transwell侵袭实验分别检测细胞迁移和侵袭情况;用细胞计数试剂盒8(CCK-8)检测细胞增殖情况。结果 对照组、川芎嗪组、川芎嗪+空载组和川芎嗪+YAP1过表达组的迁移率分别为(84.82±12.16)%、(20.67±4.48)%、(21.22±5.03)%和(76.74±0.15)%, E-cadherin蛋白相对表达水平分别为0.16±0.03、0.70±0.08、0.72±0.13和0.19±0.04,川穹嗪组上述指标与对照组比较,在统计学上差异均有统计学意义(均P<0.05);川芎嗪+YAP1过表达组上述指标与川芎嗪组、川芎嗪+空载组比较,在统计学上差异均有统计学意义(均P<0.05)。放射抵抗对照组、放射组、放射+川芎嗪组、放射+川芎嗪+空载组和放射+川芎嗪+YAP1过表达组YAP1蛋白相对表达水平分别为0.79±0.14、0.88±0.16、0.21±0.03、0.22±0.04和0.82±0.16,细胞的存活率分别为(100.00±0.00)%、(95.78±20.12)%、(40.13±6.07)%、(42.21±6.45)%和(90.12±18.65)%。放射组上述指标与放射抵抗对照组比较,在统计学上差异均有统计学意义(均P<0.05);放射+川芎嗪组、放射+川芎嗪+空载组上述指标与放射组、放射+川芎嗪+YAP1过表达组比�
Objective To study the impacts of tetramethylpyrazine(TMP)on the epithelial mesenchymal transformation and radiotherapy resistance of cervical cancer cells by regulating Yes-associated protein(YAP)/transcriptional coactivator(TAZ)signal pathway.Methods Human cervical cancer cell line C33A was randomly grouped into control group(without any intervention),TMP group(1.5 mg·mL^(-1)TMP),TMP+NC group(TMP 1.5 mg·mL^(-1)+transfection empty plasmid)and TMP+YAP1 group(TMP 1.5 mg·mL^(-1)+transfection YAP1 overexpression plasmid).C33A-RR cells of human cervical cancer were constructed and randomly separated into control-RR group(without any intervention),radiation group(6 Gy radiation),RT group(6 Gy radiation+TMP 1.5 mg·mL^(-1)),RTN group(6 Gy radiation+TMP 1.5 mg·mL^(-1)+transfection empty plasmid)and RTY group(6 Gy radiation+TMP 1.5 mg·mL^(-1)+transfection YAP1 overexpression plasmid).Western blot detected the relative expression levels of proteins;cell scratch and Transwell invasion experiments respectively examined cell migration and invasion;cell counting kit-8(CCK-8)and flow cytometry experiments respectively evaluated cell proliferation and apoptosis.Results The mobility of control group,TMP group,TMP+NC group and TMP+YAP1 group were(84.82±12.16)%,(20.67±4.48)%,(21.22±5.03)%and(76.74±0.15)%,respectively;E-cadherin protein expression levels were 0.16±0.03,0.70±0.08,0.72±0.13 and 0.19±0.04.Compared TMP group with control group of above indictors were statistically significant(all P<0.05);compared TMP+YAP1 group with TMP group and TMP+NC group were statistically significant(all P<0.05).YAP1 protein expression levels in control-RR group,radiation group,the RT group,RTN group and RTY group were 0.79±0.14,0.88±0.16,0.21±0.03,0.22±0.04 and0.82±0.16,respectively;the survival rates were(100.00±0.00)%,(95.78±20.12)%,(40.13±6.07)%,(42.21±6.45)%and(90.12±18.65)%,respectively.The difference between radiation group and control-RR group were statistically significant(all P<0.05);the differences between RT gro
作者
郭宗梁
张彩霞
张新悦
GUO Zong-liang;ZHANG Cai-xia;ZHANG Xin-yue(Department of Obstetrics and Gynecology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010059,Inner MongoliaAutonomous Region,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2024年第8期1165-1169,共5页
The Chinese Journal of Clinical Pharmacology
关键词
川芎嗪
Yes相关蛋白/转录辅激活因子
宫颈癌
上皮间质转化
放疗耐药性
tetramethylpyrazine
Yes-associated protein/transcriptional coactivator
cervical cancer
epithelial mesenchymal transformation
radiotherapy resistance
