摘要
Imaging three-dimensional,subcellular structures with high axial resolution has always been the core purpose of fluorescence microscopy.However,trade-offs exist between axial resolution and other important technical indicators,such as temporal resolution,optical power density,and imaging process complexity.We report a new imaging modality,fluorescence interference structured illumination microscopy(FI-SIM),which is based on three-dimensional structured illumination microscopy for wide-field lateral imaging and fluorescence interference for axial reconstruction.FI-SIM can acquire images quickly within the order of hundreds of milliseconds and exhibit even 30 nm axial resolution in half the wavelength depth range without z-axis scanning.Moreover,the relatively low laser power density relaxes the requirements for dyes and enables a wide range of applications for observing fixed and live subcellular structures.
基金
sponsored by the National Natural Science Foundation of China(Grant Nos.62125504,61827825,and 31901059)
STI 2030—Major Projects(Grant No.2021ZD0200401)
Major Program of the Natural Science Foundation of Zhejiang Province(Grant No.LD21F050002)
Zhejiang Provincial Ten Thousand Plan for Young Top Talents(Grant No.2020R52001)
Croucher Foundation(Grant No.CM/CT/CF/CIA/0688/19ay)
Hong Kong Innovation and Technology Fund(ITS/178/20FP and ITS/148/20).
