摘要
评价不同百合品种叶片和花对灰霉病的抗性,分析叶片和花的灰霉抗性相关性,以期为评估百合对灰霉病的抗性及百合抗病育种提供支持。以A、O、L、LA和OT等5个系列的28个百合品种为材料,利用分离鉴定的灰葡萄孢菌,采用离体菌丝块直接接种和刺伤接种法,对百合的叶片和花瓣进行灰霉病抗性评价,并根据叶和花的病斑直径进行相关性分析。结果表明,同一品种百合叶片对不同灰葡萄孢菌株抗性存在差异,不同品种间百合叶片的抗性差异较大。其中白色帝王和眼线这2个品种属于高感品种;红星、丝雨、桃色事件等8个品种属于易感品种;地魔星、秘密之吻、穿梭等7个品种属于中抗品种;索邦、微微安娜、布林迪西等6个品种属于高抗品种;依兰、西伯利亚、俯视等5个品种属于免疫品种。对叶片进行刺伤,可导致灰霉菌侵染性增强。花瓣接种结果显示大部分百合花瓣比叶片更容易受到侵染,但是百合花和叶片对灰葡萄孢的敏感性没有显著相关性;红星、丝雨、桃色事件等13个品种的花和叶对灰葡萄孢菌的敏感性一致;地魔星、秘密之吻、布林迪西等14个品种的花比叶对灰葡萄孢菌更敏感;白色帝王的叶片较花对灰葡萄孢菌更敏感。组织损伤会降低百合对灰霉病的抗性,百合叶片与花对灰霉菌敏感性无显著相关性。依兰、微微安娜、西伯利亚和红色宫殿4个高抗品种可为百合灰霉病抗性资源合理利用以及品种选育提供参考。
The resistance of leaves and flowers of different lily cultivars to gray mold was evaluated,and the correlation of leaf and flower resistance to gray mold fungi was analyzed,so as to provide some supports for evaluating lily resistance to gray mold and lily disease resistance breeding.In this study,28 lily cultivars from five series,A,O,L,LA and OT,were used as the materials,and inoculated with hyphae blocks of the isolated strains of Botrytis cinerea via direct inoculation and pricked inoculation.The results showed that the resistance of lily leaves of the same cultivar to different B.cinerea strains was different,and the resistance of lily leaves from dif-ferent cultivars was quite different.Among them,two cultivars,Watch up and Eyeliner,were high susceptible;eight cultivars,such as Trendy havana,Easy whisper,Strawbarry event,etc.were moderate susceptible;seven cultivars,including Trendy santo domingo,Secret kiss,Tresor,etc.belonged to medium resistance cultivars;six cultivars,such as Sorbonne,Viviana,Brindisi,etc.were high resistant cultivars;five varieties,like Esra,Siberia,Zeba,etc.did not show obvious symptoms in the whole vaccination cycle,were of immune cultivars.After the leaves were pricked,the infection ability of B.cinerea was increased.The results of petal inoculation showed that most lily petals were more susceptible to B.cinerea than the leaves.The correlation analysis of the flower and leaf resistance showed that there was no significant correlation between the sensitivity of lily flowers and leaves to B.cinerea.The sensitivity of the flowers and leaves of 13 cultivars to B.cinerea,such as Trendy havana,Easy whis-per and Strawbarry event,etc.was consistent;the flower sensitivity of 14 cultivars,such as Trendy santo domingo,Secret kiss and Brindisi,etc.was higer than the leaf;the leaf sensitivity of Watch up was higer than the flower.Tissue damage would reduce the resistance of lily to gray mold,and there was no significant correlation between the sensitivity of the leaf and flower to gray mold.Fou
作者
张莉雪
张紫薇
宋云泽
陈小林
段枫
鲁思勤
刘雨菡
余晓敏
朱钰
宁国贵
何燕红
ZHANG Lixue;ZHANG Ziwei;SONG Yunze;CHEN Xiaolin;DUAN Feng;LU Siqin;LIU Yuhan;YU Xiaomin;ZHU Yu;NING Guogui;HE Yanhong(National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070;The Institute of Flowers Research,Huazhong Agricultural University,Wuhan 430070;State Key Laboratory of Agricultural Microbiology,College of Plant Science and Technology,Huazhong Agricul-tural University,Wuhan 430070)
出处
《安徽农业大学学报》
CAS
CSCD
2024年第2期230-238,共9页
Journal of Anhui Agricultural University
基金
现代农业产业技术体系(CARS-23)项目资助。
关键词
百合
品种
灰葡萄孢
抗性
Lily(Lilium spp.)
cultivar
Botrytis cinerea
resistance
