摘要
为探明微生物源脂肽粗提物、代森锰锌及二者的复配剂对黄瓜枯萎病菌和葡萄白腐病菌的抑制效果,采用菌丝生长速率法、Horsfall法和Wadley公式法检测了脂肽粗提物和代森锰锌对2种病菌的抑制率和增效比。脂肽粗提物对黄瓜枯萎病菌的EC_(50)为1.93μL·mL^(-1),对葡萄白腐病菌的EC_(50)为5.54μL·mL^(-1),代森锰锌对黄瓜枯萎病菌的EC_(50)为74.37 mg·L^(-1),对葡萄白腐病菌的EC_(50)为48.45 mg·L^(-1)。Horsfall法得出脂肽与代森锰锌混配体积比为8∶2对黄瓜枯萎病菌具显著的增效活性,体积比为1∶9对葡萄白腐病菌具有显著增效活性。通过Wadley公式法得出混配组合对黄瓜枯萎病菌理论EC_(50)为1298.78 mg·L^(-1),实际EC_(50)达472.48 mg·L^(-1),增效比(SR)为2.75;对葡萄白腐病菌理论EC_(50)值为481.41 mg·L^(-1),实际EC_(50)值187.32 mg·L^(-1),增效比(SR)为2.57。结果为2种不同病害的持续防控、化学药剂的减施增效和抗菌脂肽的合理应用提供支撑。
In order to investigate the inhibitory effects of microbial crude lipopeptide extracts,mancozeb and their mixture on Fusarium oxysporum f.sp.cucumerinum and Coniella diplodiella,the inhibition rate and synergistic ratio of crude lipopeptide extracts and mancozeb on the two pathogens were detected by mycelial growth rate meth-od,Horsfall method and Wadley formula method.The results showed that the EC50 of crude lipopeptide extracts against F.oxysporum f.sp.cucumerinum was 1.93μL·mL^(-1),the EC50 of crude lipopeptide extracts against C.diplodiella was 5.54μL·mL^(-1) and EC50 of mancozeb against F.oxysporum f.sp.cucumerinum and C.diplodiella were 74.37 and 48.45 mg·L^(-1),respectively.The results of Horsfall method showed that the volume ratio of lipopep-tide to mancozeb was 8∶2,which had significant synergistic activity against F.oxysporum f.sp.cucumerinum,and the volume ratio was 1∶9,which had significant synergistic activity against C.diplodiella.According to the Wadley formula method,the theoretical EC50 of the mixture against F.oxysporum f.sp.cucumerinum was 1298.78 mg·L^(-1),the actual EC50 was 472.48 mg·L^(-1),and the synergistic ratio(SR)was 2.75;the theoretical EC50 value against C.diplodiella was 481.41 mg·L^(-1),the actual EC50 value was 187.32 mg·L^(-1),and the synergistic ratio(SR)was 2.57.The results provide some supports for the sustainable prevention and control of two different diseases,the reduction of chemical agents and the rational application of antimicrobial lipopeptides.
作者
王猛
吴含
段海明
孟祥涛
李之硕
WANG Meng;WU Han;DUAN Haiming;MENG Xiangtao;LI Zhishuo(College of Agriculture,Anhui Science and Technology University,Fengyang 233100)
出处
《安徽农业大学学报》
CAS
CSCD
2024年第2期223-229,共7页
Journal of Anhui Agricultural University
基金
安徽省自然科学基金资助项目(2308085MC90)
安徽省高校自然科学研究项目(KJ2017A510,KJ2019A0814)
作物抗逆育种与减灾国家地方联合工程实验室开放基金(NELCOF20190102)
安徽省重点研究与开发计划项目(202104a06020001)
国家级大学生创新训练计划(202210879055)共同资助。
关键词
黄瓜枯萎病
葡萄白腐病
脂肽粗提物
代森锰锌
混配
cucumber Fusarium wilt
grape white rot
crude lipopeptide extracts
Mancozeb
combination
