摘要
目的探讨核因子κb激酶亚基ε的抑制剂(IKBKE)、Yes相关蛋白1(YAP1)和转录增强结构域转录因子2(TEAD2)在结直肠癌(CRC)组织中的表达及其临床意义。方法收集2016年1月至2017年12月在蚌埠医科大学第一附属医院手术切除的142例CRC组织及对应癌旁组织,采用免疫组化法检测标本中IKBKE、YAP1和TEAD2的表达情况。分析3种蛋白在CRC组织中表达的相关性,分析蛋白阳性率与患者临床病理参数及预后的关系;绘制Kaplan-Meier生存曲线,比较这些蛋白不同表达情况患者的生存差异。采用Cox回归分析影响患者预后的危险因素。结果CRC组织中IKBKE、YAP1和TEAD2的阳性率均显著高于癌旁组织(65.5%比9.9%,73.9%比14.1%,66.9%比8.5%,均P<0.05)。IKBKE的表达与肿瘤的分化程度、浸润深度、淋巴结转移、肿瘤-淋巴结-远处转移(TNM)分期有关,YAP1和TEAD2的表达均与肿瘤的分化程度、浸润深度、淋巴结转移、远处转移及TNM分期有关。Spearman秩相关分析显示CRC组织中IKBKE与YAP1、TEAD2表达均呈正相关(均P<0.01)。Kaplan-Meier生存分析显示IKBKE、YAP1和TEAD2阳性表达组的总生存率降低。Cox回归分析显示IKBKE、YAP1和TEAD2阳性、肿瘤分化程度高、TNM分期高是CRC患者预后的独立危险因素。结论CRC中IKBKE、YAP1和TEAD2阳性表达与肿瘤的分化程度、TNM分期、转移等因素有关,可能成为CRC治疗的潜在靶点;检测这3个蛋白的表达有助于评估预后。
Objective To explore the expression and clinical significance of inhibitor of nuclear factor kappa-B kinase subunit epsilon(IKBKE),Yes-associated protein 1(YAP1),and transcriptional enhancer activation domain 2(TEAD2)in colorectal cancer tissues(CRC).Methods Paraffin-fixed samples of CRC and adjacent tissues were obtained from 142 patients at the First Affiliated Hospital of Bengbu Medical University from January 2016 to December 2017.Protein expressions of IKBKE,YAP1,and TEAD2 were detected by immunohistochemistry.The correlation of the expression of the three proteins in CRC tissues,and the relationship of positive expression rate of the proteins and clinicopathological parameters with prognosis were analyzed.The Kaplan-Meier survival curves were plotted to compare the survival differences of patients with different expression conditions of these proteins.Cox regression was used to analyze the risk factors affecting patient outcomes.Results Positive expressions of IKBKE,YAP1 and TEAD2 in CRC tissues were significantly higher than those in adjacent normal tissues(65.5%vs.9.9%,73.9%vs.14.1%,and 66.9%vs.8.5%,respectively;all P<0.05).The expressions of IKBKE,YAP1,and TEAD2 were all positively correlated with the degree of tumor differentiation,depth of invasion,lymph node metastasis,and tumor-node-metastasis(TNM)stage;while YAP1 and TEAD2 expressions were also positively correlated with distant metastasis.Spearman analysis showed that IKBKE expression was positively correlated with YAP1 and TEAD2 expressions in CRC tissues(both P<0.01).Kaplan-Meier survival analysis showed reduced overall survival for patients with positive expressions of the three proteins.Cox regression analysis revealed that positive expressions of IKBKE,YAP1,and TEAD2,higher degree of tumor differentiation,and higher TNM stage were also independent risk factors for the prognosis of CRC patients.Conclusion The positive expressions of IKBKE,YAP1 and TEAD2 in CRC are correlated with factors such as tumor differentiation,TNM stage,and metastasis.Te
作者
舒莉珊
赵洋
吴宁琪
刘梦梦
吴琼
欧玉荣
SHU Lishan;ZHAO Yang;WU Ningqi;LIU Mengmeng;WU Qiong;OU Yurong(Department of Pathology,the First Affiliated Hospital of Bengbu Medical University,Bengbu 233040,China)
出处
《浙江医学》
CAS
2024年第9期943-949,I0006,共8页
Zhejiang Medical Journal
基金
安徽省教育厅项目(2022AH051505)。
关键词
结直肠癌
核因子κb激酶亚基ε的抑制剂
Yes相关蛋白1
转录增强结构域转录因子2
预后
Colorectal cancer
Inhibitor of nuclear factor kappa-B kinase subunit epsilon
Yes-associated protein 1
Transcriptional enhancer activation domain 2
Prognosis
