摘要
目的细胞温度成像可以帮助科学家研究和理解细胞内部的温度分布,揭示细胞代谢和生物化学过程的关键信息。目前,基于荧光温度探针的细胞温度成像技术存在低温度分辨率和有限测量范围等限制。本文旨在利用单分子量子相干过程依赖温度的特性,开发一种单细胞温度成像和实时检测技术。方法基于飞秒脉冲激光制备延时和相位可调的飞秒脉冲对,调制的脉冲对通过显微系统激发细胞内标记的荧光单分子,之后收集并记录每个荧光光子的到达时间。利用单分子相干过程与周围环境温度的关系,定义单分子量子相干可视度(V),建立V与环境温度的对应关系。通过调制解调荧光光子的到达时间,获取单分子周围环境温度,结合扫描成像,实现细胞的温度成像和实时检测。结果该方法可以实现高精度(温度分辨率<0.1℃)和大范围温度(10~50℃)的温度成像和测量,并观测到了单个细胞代谢相关的温度变化。结论该研究有助于深入了解细胞代谢、蛋白质功能和疾病机制,为生物医学研究提供重要工具。
Objective Cellular temperature imaging can assist scientists in studying and comprehending the temperature distribution within cells,revealing critical information about cellular metabolism and biochemical processes.Currently,cell temperature imaging techniques based on fluorescent temperature probes suffer from limitations such as low temperature resolution and a limited measurement range.This paper aims to develop a single-cell temperature imaging and real-time monitoring technique by leveraging the temperature-dependent properties of single-molecule quantum coherence processes.Methods Using femtosecond pulse lasers,we prepare delayed and phase-adjustable pairs of femtosecond pulses.These modulated pulse pairs excite fluorescent single molecules labeled within cells through a microscopic system,followed by the collection and recording of the arrival time of each fluorescent photon.By defining the quantum coherence visibility(V)of single molecules in relation to the surrounding environmental temperature,a correspondence between V and environmental temperature is established.By modulating and demodulating the arrival times of fluorescent photons,we obtain the local temperature of single molecules.Combined with scanning imaging,we finally achieve temperature imaging and real-time detection of cells.Results This method achieves high precision(temperature resolution<0.1℃)and a wide temperature range(10-50℃)for temperature imaging and measurement,and it enables the observation of temperature changes related to individual cell metabolism.Conclusion This research contributes to a deeper understanding of cellular metabolism,protein function,and disease mechanisms,providing a valuable tool for biomedical research.
作者
周海涛
秦成兵
肖连团
武志芳
李思进
ZHOU Hai-Tao;QIN Cheng-Bing;XIAO Lian-Tuan;WU Zhi-Fang;LI Si-Jin(Department of Nuclear Medicine,The First Hospital of Shanxi Medical University,Collaborative Innovation Center for Molecular Imaging,Shanxi Medical University,Taiyuan 030001China;Collaborative Innovation Center of Extreme Optics,Institute of Laser Spectroscopy,State Key Laboratory of Quantum Optics and Quantum Optics Devices,Shanxi University,Taiyuan 030006,China)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2024年第5期1215-1220,共6页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金(62005150,81971655,62127817,62222509,U22A6008,U22A2091)资助项目。
关键词
量子相干调制
单分子显微
细胞温度成像
quantum coherent modulation
single-molecule microscopy
cellular temperature imaging
