摘要
目的利用斑马鱼为动物模型,初步分析口腔炎合剂对胚胎及肝肾发育的影响。方法待斑马鱼发育至受精后10 h(10 hpf)时以简单随机方法分组并添加梯度浓度的口腔炎合剂。药物作用72 h后在显微镜下观察口腔炎合剂对胚胎及肝肾发育,并记录图像数据。结果当口腔炎合剂稀释浓度高于1.625×10^(-5)g/mL时对10 hpf斑马鱼开始出现胚胎发育毒性,表现为发育迟缓、心腔狭窄等。当口腔炎合剂稀释浓度高于6.500×10^(-5)g/mL时对36 hpf斑马鱼产生肝肾发育毒性,主要表现为肝脏与周边组织分界线不太明显,肾脏和鱼鳔区域明显增大,并伴有水肿瘀滞现象。结论口腔炎合剂具有一定的胚胎及肝肾毒性,孕妇和哺乳期妇女,以及肝肾功能不全者原则上不得使用本品,必须使用时应严格限量,并在医生指导下谨慎使用。
Objective To preliminarily analyze the effect of stomatitis mixture on embryo,liver and kidney development by using zebrafish as an animal model.Methods When zebrafish developed to 10 h after fertilization(10 hpf),they were grouped by simple random method and added with gradient concentration of stomatitis mixture.After 72 h of drug action,the toxicity of stomatitis mixture on embryonic development and hepat orenal toxicity were observed under microscope and the image data were recorded.Results When the dilution concentration of stomatitis mixture was higher than 1.625×10^(-5)g/mL,embryo developmental toxicity began to appear in 10hpf zebrafish,showing developmental delay and cardiac cavity stenosis.When the dilution concentration of stomatitis mixture was higher than 6.500×10^(-5)g/mL,it had hepatorenal toxicity to 36 hpf zebrafish.The main manifestations were that the boundary between liver and surrounding tissues was not obvious,and the kidney and swim bladder area increased significantly,accompanied by edema and stasis.Conclusion The stomatitis mixture has certain embryo and liver and kidney toxicity.Pregnant women,lactating women,and patients with liver and kidney dysfunction,are not allowed to use this product in principle.It must be used with strict limits and careful use under the guidance of doctors.
作者
杨波
崔建蓉
王娅俐
曹治兴
袁晓燕
YANG Bo;CUI Jianrong;WANG Yali;CAO Zhixing;YUAN Xiaoyan(Chengdu Seventh People′s Hospital,Chengdu,Sichuan 610213,China;Nanchong Central Hospital,Nanchong,Sichuan 637000,China;School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan 611137,China;Panzhihua Central Hospital,Panzhihua,Sichuan 617067,China)
出处
《现代医药卫生》
2024年第9期1446-1449,1455,共5页
Journal of Modern Medicine & Health
基金
四川省医学科研课题计划项目(S23074)
四川省自然科学基金项目(2022NSFSC1535)。
关键词
口腔炎合剂
模式生物
斑马鱼
肝肾毒性
胚胎毒性
Stomatitis mixture
Model organism
Zebrafish
Hepatorenal toxicity
Embryo toxicity
