摘要
为解析夏季环境胁迫和长牡蛎机体响应特征,实验针对2021年北黄海夏季高温降雨期的长牡蛎养殖海区开展了2次调查研究和对比分析。结果显示,7月海区表层水温分别为25.3和24.9℃,盐度分别为26.41和27.87;表层海水中硅藻丰度分别为3.6×10^(3)和3×10^(3)个/L,低于往年平均水平,甲藻多样性增加,表层海水中细菌的总丰度分别为2.10×10^(9)和2.77×10^(10)个/L,弧菌丰度分别为3.37×10^(8)和5.40×10^(8)个/L;长牡蛎肝胰腺糖原含量分别为40.96和31.58 mg/g、闭壳肌糖原含量分别为6.63和8.91 mg/g;鳃丙二醛(MDA)含量分别为13.74和8.69 nmol/mg prot、鳃总抗氧化能力(T-AOC)分别为0.46和0.77 U/mg prot;海区养殖长牡蛎血淋巴细胞中CgIL17-5和CgTNF-1的mRNA表达量在第一次调查时相对室内暂养长牡蛎均剧烈升高;血淋巴细胞中CgCaspase3的mRNA表达量升高但差异不显著。研究表明,2021年北黄海贝类养殖区在经历6—7月夏季高温降雨期后出现水温升高和盐度降低现象,硅藻丰度较往年平均水平下降而甲藻多样性增加,细菌总丰度较入夏前水平降低而弧菌丰度显著升高。与室内暂养对照组长牡蛎相比,海区养殖长牡蛎的糖原含量下降,并发生轻度氧化应激。研究结果有助于进一步理解夏季养殖环境的变化规律和长牡蛎机体响应特征,为预防夏季大规模死亡发生提供理论依据和参考。
Summer mass mortality is one of the major bottlenecks limiting the green and high-quality development of the Pacific oyster Crassostrea gigas aquaculture in China.The frequent environmental stresses in summer,including high-temperature,rainfall,pathogen stimulation,picophytoplankton abundance and reproductive behavior,are important causes of mass mortality.Monitoring the changes of key environmental factors and healthrelated indicators in the aquaculture of C.gigas will help to forecast the risk of mass mortality.In this study,two surveys were conducted for C.gigas aquaculture in the North Yellow Sea during the summer heat and rainfall period to monitor the variations of water parameters,picophytoplankton abundance,bacterial abundance,glycogen content and immune-related indicators.It was found that,for the first and second survey in July,the surface water temperature was 25.3 and 24.9℃,respectively.The salinity for the two surveys was 26.41 and 27.87,respectively.The abundance of diatoms for the two surveys was 3.6×10^(3)and 3×10^(3)cells/L,respectively,which was lower than the average level of previous reports,and the diversity of dinoflagellates increased.For the two surveys,the abundance of total bacteria in the water was 2.10×10^(9)and 2.77×10^(10)copies/L,respectively,and the abundance of Vibrio was 3.37×10^(8)and 5.40×10^(8)copies/L,respectively.The glycogen content of hepatopancreas decreased to 40.96 and 31.58 mg/g respectively for the two surveys,and the glycogen content of adductor muscle decreased to 6.63 and 8.91 mg/g,respectively.MDA content in gill was 13.74 and 8.69 nmol/mg prot for the two surveys,respectively,and T-AOC in gill was 0.46 and 0.77 U/mg prot,respectively.The expression levels of CgIL17-5 and CgTNF-1 mRNA in haemocytes dramatically increased in the first survey compared to the oysters cultured indoor.The expression levels of CgCaspase3 mRNA in haemocytes increased compared to the oysters cultured indoor,but the differences were not significant.In conclusion,the results showed
作者
高磊
孔宁
刘冉阳
赵俊彦
邢镇
张子杨
赵宝
李庆嵩
付强
王文彪
李磊
王玲玲
宋林生
GAO Lei;KONG Ning;LIU Ranyang;ZHAO Junyan;XING Zhen;ZHANG Ziyang;ZHAO Bao;LI Qingsong;FU Qiang;WANG Wenbiao;LI Lei;WANG Lingling;SONG Linsheng(Liaoning Key Laboratory of Marine Animal Immunology and Disease Control,Liaoning Key Laboratory of Marine Animal Immunology,Dalian Ocean University,Dalian 116023,China;Dalian Yuyang Group Co.,Ltd.,Dalian 116499,China)
出处
《水产学报》
CAS
CSCD
北大核心
2024年第4期159-170,共12页
Journal of Fisheries of China
基金
国家重点研发计划(2018YFD0900606)
国家自然科学基金国际(地区)合作与交流项目(41961124009)
农业农村部:国家现代农业产业技术体系(CARS-49)
大连市科技创新基金(2021JJ13SN73)
辽宁省应用基础研究计划(2022JH2/101300140)。
关键词
长牡蛎
高温
降雨
糖原
免疫
北黄海
Crassostrea gigas
high temperature
rainfall
glycogen
immune
North Yellow Sea
