摘要
目的研究miR-299-5p对头颈部鳞癌(HNSCC)早期诊断及预后的价值。方法选取从2018年1月至2019年1月在我院收治的HNSCC患者65例,在手术过程中收集患者癌组织和癌旁组织。采用荧光定量PCR检测癌组织和癌旁组织miR-299-5p水平;采用受试者工作特征(ROC)曲线分析miR-299-5p对HNSCC的早期诊断;采用Kaplan-Meier法绘制HNSCC患者生存曲线;采用Cox回归分析HNSCC患者预后的影响因素。结果与癌旁正常组织相比,HNSCC患者癌组织中miR-299-5p水平明显降低(P<0.05);HNSCC患者癌组织miR-299-5p水平与TNM分期、HPV感染、分化程度、有无淋巴结转移相关(P<0.05);miR-299-5p水平对HNSCC患者的早期诊断的AUC为0.825,灵敏度为88.51%,特异性为73.83%,截断值为0.852;Kaplan-Meier生存曲线分析结果显示miR-299-5p低表达组3年生存率(9/32,28.13%)明显低于miR-299-5p高表达组(23/33,63.89%)(χ^(2)=18.698,P=0.003);单因素Cox回归分析表明,miR-299-5p低表达、HPV感染、淋巴结转移均是影响HNSCC患者不良预后的危险因素(P<0.05);多因素Cox回归分析表明,miR-299-5p低表达、HPV感染、淋巴结转移是影响HNSCC患者不良预后的独立危险因素(P<0.05)。结论HNSCC患者癌组织中miR-299-5p水平明显低于癌旁组织,且其对于HNSCC的早期诊断和预后具有较高的价值。
Objective To evaluate the value of miR-299-5p in the early diagnosis and prognosis of head and neck squamous cell carcinoma(HNSCC).Methods A total of 65 patients with HNSCC who were admitted to our hospital from January,2018 to January,2019 were selected for the study.Cancer tissues and adjacent tissues of these patients were collected during the operation.Fluorescence quantitative PCR was used to detect the level of miR-299-5p in cancer tissues and adjacent tissues.The receiver operating characteristic(ROC)curve was drawn to analyze the early diagnosis of HNSCC by miR-299-5p.Kaplan-Meier method was used to plot the survival curve of HNSCC patients.Cox regression was used to identify the influencing factors of prognosis of patients with HNSCC.Results Compared with adjacent normal tissues,the level of miR-299-5p in cancer tissues of HNSCC patients was significantly reduced(P<0.05).The level of miR-299-5p in cancer tissue of HNSCC patients was correlated with TNM stage,HPV infection,degree of differentiation,and presence or absence of lymph node metastasis(P<0.05),and the AUC of miR-299-5p level for the early diagnosis of HNSCC patients was 0.825,with the sensitivity of 88.51%,and the specificity of 73.83%.The cut-off value was 0.852,and Kaplan-Meier survival curve analysis showed that the 3-year survival rate of miR-299-5p low expression group(9/32,28.13%)was significantly lower than that of miR-299-5p high expression group(23/33,63.89%)(χ^(2)=18.698,P=0.003).Univariate Cox regression analysis showed that low expression of miR-299-5p,HPV infection and lymph node metastasis were risk factors for the poor prognosis in patients with HNSCC(P<0.05),and multivariate Cox regression analysis showed that low expression of miR-299-5p,HPV infection and lymph node metastasis were independent risk factors for the poor prognosis in patients with HNSCC(P<0.05).Conclusion The level of miR-299-5p in cancer tissues of patients with HNSCC is significantly lower than that in adjacent tissues,and it has a high value for the early dia
作者
刘丽莎
贾巧静
王建星
张艳茹
马利娜
张海中
LIU Lisha;JIA Qiaojing;WANG Jianxing;ZHANG Yanru;MA Lina;ZHANG Haizhong(Department of Otolaryngology Head and Neck Surgery,ShijiazhuangSixth Hospital,Shijiazhuang 050050,China;Department of Otolaryngology Head and Neck Surgery,the Second Hospital of Hebei Medical University,Shijiazhuang 050004,China)
出处
《标记免疫分析与临床》
CAS
2024年第4期679-684,共6页
Labeled Immunoassays and Clinical Medicine
基金
河北省卫生和计划生育委员会科研基金项目(编号:20222773)。
关键词
MiR-299-5p
头颈部鳞癌
早期诊断
预后
Micro RNA-299-5p
Squamous cell carcinoma of head and neck
Early diagnosis
Prognosis
