摘要
目的:探究大花杓兰根内与根际土壤内生真菌的组成与相关性,为其种群恢复和资源可持续利用提供理论依据。方法:采用高通量测序技术对大花杓兰根内与根际土壤真菌的核基因ITS2片段进行扩增测序。结果:除极个别样本外,在OTU、属、科、目、纲和门等分类级别,大花杓兰根部内生真菌数量与根际土壤真菌数量差异较大,所有根际土壤样本内生真菌数量均大于根样本。不同样本间无共有OTUs和属。Alpha多样性结果显示,根际土壤的真菌群落丰富度大于根样本,但多样性差异不明显。在科水平上,根与根际土壤无共有优势菌。PCoA主成分分析和UPGMA聚类分析结果显示,根样本与根际土壤样本在组成上存在差异。在真菌的特异性组成方面,Rozellomycota真菌在根样本和根际土壤样本中相对丰度接近,分布较为均匀,Malassezia真菌只在植物根样本中检测到,为植物根样本的特有真菌,蜡壳耳属(Sebacina)只在根际土壤中检测到,为根际土壤的特有属。结论:大花杓兰根与其根际土壤真菌群落组成存在差异性,根际土壤真菌的丰富度大于根样本,二者真菌群落相关性小,无共有优势菌科。
Objective:The composition and correlation of endophytic fungi in the roots and rhizosphere soil of Cypripedium macranthos was analyzed to provide theoretical basis for the field population recovery and sustainable resource utilization.Methods:The nuclear gene ITS2 fragment of endophytic fungi in the roots and rhizosphere soil was amplified and sequenced by high-throughput sequencing.Results:Except for a few samples,the number of endophytic fungi in OTU,genus,family,order,class and phylum was significantly different in roots and rhizosphere soil,and the number of endophytic fungi in all rhizosphere soil samples were larger than that in root samples.There was no common OTU and genus among the different samples.The results of Alpha diversity showed that the richness of fungal community in rhizosphere soil was higher than that in root samples,but the difference in diversity was not significant.At the family level,there were no common dominant fungi in root and rhizosphere soil.The results of PCoA principal component analysis and UPGMA cluster analysis showed that there were differences in composition between root and rhizosphere soil samples.In terms of the specific composition of fungi,Rozellomycota was relatively close in abundance and relatively uniform in root samples and rhizosphere soil samples.Malassezia was only detected in root samples and was endemic to root samples.Sebacina was only detected in rhizosphere soil and was endemic to rhizosphere soil.Conclusion:The endophytic fungal community composition of roots and rhizosphere soil was different.The abundance of endophytic fungi in rhizosphere soil was higher than that in root samples.The correlation between the two fungal communities was small,and there was no common dominant fungi.
作者
樊杰
李冲冲
齐旭超
黄啟永
任玉亮
刘计权
杜晨晖
Fan Jie;Li Chongchong;Qi Xuchao;Huang Qiyong;Ren Yuliang;Liu Jiquan;Du Chenhui(College of Traditional Chinese Medicine and Food Engineering,Shanxi University of Traditional Chinese Medicine,Jinzhong 030619,China)
出处
《中国野生植物资源》
CSCD
2024年第4期59-67,共9页
Chinese Wild Plant Resources
基金
山西省中医药管理局项目(2020ZYYC002)
山西中医药大学青苗项目(2018PY-017)
山西道地药材品质形成机制创新团队(2022TD2009)
山西中医药大学科技创新能力培育计划“太行本草”专项(2022PY-TH-27)
山西省教育厅教改项目(J20220798)。
关键词
大花杓兰
根内真菌
根际土壤
高通量测序
Cypripedium macranthos
Fungi in the root
Rhizosphere soil
High-throughput sequencing
