摘要
为了探究焦脱镁叶绿酸a(PPa)对植物病原菌的抑制作用,以黄瓜枯萎病病原菌尖孢镰刀菌黄瓜专化型菌(Fusarium oxysporium f.sp.cucumerinum)H_(46)5菌和‘长春密刺’黄瓜为材料,测定不同浓度PPa对菌丝生长的影响,以及经不同强度和时长光照的PPa对感枯萎病的黄瓜叶片的病斑面积,丙二醛和游离脯氨酸含量,过氧化物酶、过氧化氢酶和超氧化物歧化酶活性及活性氧的影响。结果表明:PPa对H_(46)5菌株生长有明显抑制作用,其抑菌效果与浓度呈正相关,浓度为2 g·L^(-1)效果最佳。PPa具有光敏性,其经适当强度和时长的光照后喷施感染枯萎病的黄瓜叶片,可显著降低叶片感病程度与MDA含量,提升脯氨酸水平与抗氧化能力,维持活性氧平衡。上述结果表明,PPa具有很好的防治黄瓜枯萎病效果。
In order to explore the inhibitory effect of pyropheophorbide-a(PPa)on plant pathogens,and to find a new agent with low toxicity and high efficiency against the cucumber Fusarium wilt(CFW),Fusarium oxysporium f.sp.cucumerinum H_(46)5 strain and cucumber cultivar‘Changchun Mici’were used as materials,and the effect of different concentrations of PPa on the mycelial growth of H_(46)5 pathogen was deteced.The area of infected cucumber leaves,the malondialdehyde(MDA)content,the free proline content were measured.The activities of peroxidase(POD),catalase(CAT)and superoxide dismutase(SOD)were determined after PPa treatment under different lighting condition.The reactive oxygen species(ROS)including H_(2)O_(2)content and superoxide anion production rate was estimated using 3,3′-diaminobenzidine(DAB)staining and nitro-blue tetrazolium(NBT)staining.The results showed that PPa had a significant inhibitory effect on H_(46)5,and its inhibitory effect was positively correlated with its concentration.The optimal inhibitory concentration of PPa was 2 g·L^(-1).PPa treatment also significantly reduced the level of CFW disease and MDA content of the plant,and increased the proline content and antioxidant capacity of the plant,and maintained the ROS balance of the plant.Although the inhibitory ability of PPa was limited by the effects of light intensity and light duration,and the results proved that PPa had a good effect on inhibiting the disease of CFW.
作者
于静
冯向君
金英学
丁国华
YU Jing;FENG Xiangjun;JIN Yingxue;DING Guohua(School of Life Science&Technology,Harbin Normal University,Harbin 150025,China;School of Chemistry&Chemical Engineering,Harbin Normal University,Harbin 150025,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2024年第4期859-874,共16页
Acta Horticulturae Sinica
基金
黑龙江省自然科学基金重点项目(ZD2018001)。
关键词
黄瓜
枯萎病
焦脱镁叶绿酸a
活性氧
抗病
cucumber
Fusarium wilt
pyropheophorbide-a
reactive oxygen species
disease resistance
