摘要
利用34个柑橘黄龙病菌亚洲种“Candidatus Liberibacter asiaticus”(CLas)基因组进行泛基因组(Pan-genome)构建,并对其进化和拷贝数变异(Copy number variations,CNV)等方面进行了深入分析。结果表明此泛基因组共含有36593个基因,1188个基因簇,其中核心基因簇有872个、可变基因簇271个和个体特有基因簇45个。对所有基因簇进行选择压力分析,发现159个基因簇经历了适应性进化。结合GO注释数据,本研究中挖掘出CLas中两个基因可能与致病性或治疗耐药性相关。对所有的基因簇进行拷贝数进行统计,同时深入分析其中8个基因簇,结果发现其中5个基因簇与CLas的致病性或抗性有关,因此推测拷贝数增加的基因可能与抗药性相关。
In this work,a pan-genome from 34 genomes of Candidatus Liberibacter asiaticus(CLas)was constructed and the extensive analyses of evolution and copy number variations were completed.The CLas pan-genome was composed of 36593 genes and 1188 gene clusters,including 872 core gene clusters,271 accessory gene clusters,and 45 strain-specific gene clusters.An examination for selection pressures of all gene clusters showed that 159 of them underwent adaptive evolution.Two genes associated with pathogenicity or treatment resistance in CLas were mined in combination with GO annotation data.An in-depth analysis of copy number variations in eight genes revealed that five genes were connected to pathogenicity or resistance of CLas.This result revealed that CLas develops resistance to antiseptics by increasing the copy number of relevant genes.
作者
熊志伟
李智龙
尹晖
高玉霞
XIONG Zhiwei;LI Zhilong;YIN Hui;GAO Yuxia(Department of Navel Orange,Gannan Normal University,Ganzhou,Jiangxi 341000,China;The National Navel Orange Engineering and Technology Research Center,China-USA Citrus Huanglongbing Joint Laboratory,Ganzhou,Jiangxi 341000,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2024年第4期737-747,共11页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(32060615)
江西省自然科学基金项目(20202BABL213049)
江西省“双千计划”项目(jxsq2020102134)。
关键词
柑橘
黄龙病菌
泛基因组
适应性进化
拷贝数变异
citrus
Huanglongbing
pan-genome
adaptive evolution
copy number variation
