摘要
目的掌握辽宁省水产动物源副溶血性弧菌的耐药情况,探讨耐药基因型与表型间的关联性,进一步揭示耐药机制,为科学防控副溶血性弧菌食物中毒提供理论依据。方法应用肉汤稀释法对168株水产动物源副溶血性弧菌进行耐药谱研究,测定菌株对14种常见抗生素的最小抑菌浓度和其中4种抗生素的最小杀菌浓度。应用实时荧光定量PCR方法对菌株进行7种常见耐药基因及I类整合子inT1基因的检测。结果168株辽宁省水产动物源副溶血性弧菌对14种常见抗生素的耐药率分别为青霉素G 98.21%、磺胺甲噁唑70.24%和氨苄西林67.85%。菌株对恩诺沙星的敏感率最高,为97.62%,且出现不同耐药程度的多重耐药株,AMP/PG/SMX和PG/SMX为优势耐药菌谱。菌株主要耐药基因型有blaCARB(29.55%)、blaCARB-Sul II(15.26%)、blaCARB-blaTEM(15.26%)。耐药基因与耐药表型的符合率不高。结论水产动物源副溶血性弧菌对磺胺甲噁唑和β-内酰胺类抗生素耐药率较高,对恩诺沙星的敏感率最高,恩诺沙星的杀菌和抑菌效果显著,提示可将恩诺沙星用于预防和治疗副溶血性弧菌病的首选药。水产动物源副溶血性弧菌的耐药严重,多重耐药株数量日益增多。众多因素可影响副溶血性弧菌对抗生素的耐药表型,而且大多数耐药基因可在副溶血性弧菌间水平传播,从而增加了对水产动物源副溶血性弧菌病的防治难度。相关部门有必要加强对副溶血性弧菌耐药基因型及表型的监测,进一步揭示其耐药机制,为科学防控水产动物源副溶血性弧菌病提供理论依据。
Objective To master the drug resistance of Vibrio parahaemolyticus from aquatic animals in Liaoning Province,explore the correlation between drug resistance gene and phenotype,and further reveal the drug resistance mechanism,so as to provide a theoretical basis for scientific prevention and control of Vibrio parahaemolyticus food poisoning.Methods The drug resistance spectrum of 168 strains of Vibrio parahaemolyticus from aquatic animals was studied by broth dilution method.The minimum inhibitory concentration of the strains against 14 common antibiotics were determined.The minimum bactericidal concentrationof the strains against 4 common antibiotics were determined.7 common drug resistance genes and class Ⅰ integron inT1 gene were detected by real-time PCR.Results The drug resistance rates of 168 strains of Vibrio parahaemolyticus from aquatic animals in Liaoning province to 14 common antibiotics were 98.21% of penicillin G,70.24% of sulfamethoxazole and 67.85% of ampicillin,respectively.The sensitivity rate of Vibrio parahaemolyticus from aquatic animals in Liaoning Province to enrofloxacin was the highest(97.62%).168 strains of VP showed multiple drug resistance with different degrees of drug resistance,and AMP/PG/SMX and PG/SMX were the dominant drug-resistant strains.The dominant genotypes were blaCARB(29.55%),blaCARB-blaTEM(15.26%)and blaCARB-Sul II(15.26%).The coincidence rate between drug resistance genes and drug resistance phenotypes was not high.Conclusions Vibrio parahaemolyticus derived from aquatic animals had higher resistance rate to sulfamethoxazole and β-lactam antibiotics,and the highest sensitivity rate to Enrofloxacin.Enrofloxacin had significant bactericidal and bacteriostatic effects,suggesting that Enrofloxacin could be used as the first choice for prevention and treatment of vibrio parahaemolyticus disease.The resistance of Vibrio parahaemolyticus from aquatic animals is severe,and the number of multidrugresistant strains is increasing.Many factors can affect the antibiotic resistance
作者
李雪
王伟杰
孙婷婷
魏彤竹
雷露
LI Xue;WANG Wejjie;SUN Tingting;WEI Tongzhu;LEI Lu(Inspection and Testing Institute,Liaoning Provincial Center for Disease Control and Prevention,Shenyang,Liaoning,110005,China)
出处
《职业与健康》
CAS
2024年第4期461-465,共5页
Occupation and Health
基金
辽宁省自然科学基金项目(2023-MS-064)。
关键词
辽宁省
水产动物源副溶血性弧菌
耐药性
耐药基因
耐药表型
Liaoning Province
Vibrio parahaemolyticus of aquatic animal origin
Drug resistance
Drug resistance gene
Drug resistance phenotype
