摘要
目前,我国工业大麻种质资源的分子筛选和分子鉴定主要依赖于AFLP、SSR和重测序等方法,缺乏快速、准确、直观的分子标记体系,开发竞争性等位基因特异性PCR(Kompetitive Al-lele Specific PCR,KASP)标记集及其检测体系具有良好的应用前景。研究首先基于大麻素B位点基因多态性成功开发了1个特异性KASP位点,能够准确区分大麻种质资源中的低毒型、高毒型和中间型三种化学型;随后基于来自95份不同大麻种质资源的全基因组重测序SNP数据,总共开发了32个高质量的核心KASP标记。验证表明,该33个KASP标记集及其检测体系可以应用于工业大麻辅助育种、工业大麻品种分子鉴别和指纹图库构建等,丰富了工业大麻分子标记类型和方法。
Currently,the molecular screening and identification of industrial hemp germplasm re-sources in China mainly rely on methods such as AFLP,SSR and Resequencing,lacking in a fast,accu-rate,and intuitive molecular marker system.The development of Kompetitive Allele Specific PCR(KASP)with marker sets and its detection system has good application prospects.This study first suc-cessfully developed a specific KASP locus based on the cannabinoid B locus gene polymorphism,which can accurately distinguish different chemical types of low-THC,high-THC or intermediate types in can-nabis germplasm resources.A total of 32 high-quality core KASP markers were developed based on the whole-genome resequencing single nucleotide polymorphism(SNP)data from 95 different cannabis germ-80 plasm resources.Verification experiment showed that the 33 KASP marker sets and its detection system can be applied to industrial hemp assisted breeding,molecular identification of industrial hemp varieties,and fingerprint library construction,enriching the types and methods of industrial hemp molecular markers.
作者
周佳
张园
郭孟璧
吕品
杜光辉
许艳萍
杨明
张庆滢
字雪靖
陈璇
ZHOU Jia;ZHANG Yuan;GUO Mengbi;LYU Pin;DU Guanghui;XU Yanping;YANG Ming;ZHANG Qingying;ZI Xuejing;CHEN Xuan(School of Agriculture,Yunnan University,Kunming 650504,Yunnan,China;Industrial Crops Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,Yunnan,China)
出处
《中国麻业科学》
2024年第2期80-89,共10页
Plant Fiber Sciences in China
基金
财政部和农业农村部:国家麻类产业技术体系育种技术与方法岗位(CARS-16-E02)
云南省科技计划项目(202305AP350011)
云南省高层次人才培养支持计划(青年拔尖)。
关键词
工业大麻
种质鉴定
大麻素
KASP标记
指纹图谱
industrial hemp
germplasm identification
cannabinoid
KASP marker
fingerprint
