摘要
目的探讨2型糖尿病患者血清LncRNA MEG3水平与糖尿病肾病的相关性。方法选取140例2型糖尿病患者,依据尿微量白蛋白/肌酐比值(UACR)分为正常蛋白尿组(UACR<30 mg/g)45例、微量蛋白尿组(UACR 30~300 mg/g)47例、大量蛋白尿组(UACR>300 mg/g)48例。选取同期于本院进行健康体检的50例健康人群为对照组。收集2型糖尿病患者临床资料,实时荧光定量PCR(qRT-PCR)检测患者血清LncRNA MEG3水平,分析血清LncRNA MEG3水平与临床指标的相关性及其对糖尿病肾病的诊断价值。结果相较于对照组,正常蛋白尿组、微量蛋白尿组及大量蛋白尿组患者血清LncRNA MEG3表达均显著上调(P<0.05)。微量蛋白尿组和大量蛋白尿组空腹血糖(FPG)、空腹胰岛素(FIns)、胰岛素抵抗指数(HOMA-IR)、糖化血红蛋白(HbA1c)、收缩压(SBP)、血肌酐(SCr)、尿微量白蛋白/肌酐比值(UACR)、转化生长因子-β1(TGF-β1)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及血清LncRNA MEG3高于正常蛋白尿组,且大量蛋白尿组高于微量蛋白尿组(P<0.05)。大量蛋白尿组舒张压(DBP)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)高于正常蛋白尿组(P<0.05)。血清LncRNA MEG3水平与T2DM病程、FPG、FIns、HOMA-IR、HbA1c、SBP、DBP、TC、LDL-C、SCr、UACR、IL-6、TNF-α、TGF-β1呈正相关(P<0.05)。血清LncRNA MEG3水平诊断糖尿病肾病的AUC为0.960,以1.5为阈值,其诊断的灵敏度为94.74%、特异度为84.44%。结论糖尿病肾病患者血清LncRNA MEG3水平明显升高,且与糖脂代谢、胰岛素抵抗及炎症因子相关,对糖尿病肾病具有较好的诊断价值。
Objective To explore the correlation between serum LncRNA MEG3 level and diabetic nephropathy(DN)in patients with type 2 diabetes mellitus(T2DM).Methods One hundred and forty T2DM patients were selected.They were divided into a normal albuminuria group(n=45;UACR<30 mg/g),a microalbuminuria group(n=47;UACR 30~300 mg/g)and a large al-buminuria group(n=48;UACR>300 mg/g)according to urinary microalbuminuria/creatinine ratio(UACR).Fifty healthy people who underwent physical examination in our hospital during the same period were selected as a control group.Clinical data of T2DM patients were collected.Serum LncRNA MEG3 levels were detected by real-time quantitative fluorescent PCR(qRT-PCR).The correlation between serum LncRNA MEG3 levels and clinical indicators as well as the diagnostic value of serum LncRNA MEG3 in DN were analyzed.Results Compared with the control group,the expression of serum LncRNA MEG3 in the normal albuminuria group,the microalbuminuria group and the macroalbuminuria group was significantly up-regulated(P<0.05).Fasting blood glucose(FPG),fasting insulin(FIns),insulin resistance index(HOMA-IR),glycosylated hemoglobin(HbA1c),systolic blood pressure(SBP),blood creatinine(SCr),urinary UACR,transforming growth factor-β1(TGF-β1),Interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and serum LncRNA MEG3 in the microalbuminuria group and the macroalbuminuria group were higher than those in the normal albuminuria group,and those in the macroalbuminuria group were higher than those in the microalbuminuria group(P<0.05).The diastolic blood pressure(DBP),total cholesterol(TC)and low density lipoprotein cholesterol(LDL-C)in the mac-roalbuminuria group were higher than those in the normal albuminuria group(P<0.05).Serum LncRNA MEG3 levels were positively correlated with the course of T2DM,FPG,FIns,HOMA-IR,HbA1c,SBP,DBP,TC,LDL-C,SCr,UACR,IL-6,TNF-αand TGF-β1(P<0.05).The AUC of serum LncRNA MEG3 level for the diagnosis of DN was 0.960(95%CI=0.913~0.986),and the diagnostic sensitivity and specificity were 94.
作者
徐丽玲
黄剑良
XU Li-ling;HUANG Jian-liang(Department of General Medicine,Hechi People's Hospital,Hechi 547000,China)
出处
《实用医院临床杂志》
2024年第3期155-159,共5页
Practical Journal of Clinical Medicine
基金
广西壮族自治区卫生健康委员会自筹经费科研课题(编号:Z20210319)。
