摘要
目的基于ADP-heptose/ALPK1探讨阴道微生态对HPV感染的作用机制。方法收集2020年1月至2023年10月84例HPV阳性宫颈癌患者(观察组),107例无宫颈癌的HPV阳性患者(阳性组),191名健康女性(对照组)。收集患者阴道分泌物,赖特-吉姆萨染色检测乳酸菌水平和微生物群多样性和密度;将另一支拭子置于0.9%氯化钠溶液中,用于测量分泌物的清洁度和念珠菌,滴虫和乳酸杆菌的水平;第三拭子用于检测阴道分泌物的pH值。生物信息学分析阴道菌群情况,qRT-PCR检测ADP-heptose/ALPK1 mRNA水平。结果3组间菌群Alpha多样性指标:Chao1指数(F=32.780,P<0.05)、Ace指数(F=6.270,P<0.05)、Shannon指数(F=17.240,P<0.01)、Simpson指数(F=9.710,P<0.05)差异均有统计学意义。3组间伯克霍尔德菌及乳杆菌属相对丰度差异有统计学意义(P<0.05),观察组与阳性组伯克霍尔德菌属相对丰度高于对照组(χ^(2)=2.18,P=0.03),而乳杆菌属相对丰度低于对照组(χ^(2)=3.13,P<0.01)。LefSe分析显示,观察组、阳性组和对照组之间4个特征菌属的丰度有差异(LDA值>2.0)。观察组与阳性组菌群中伯克霍尔德菌和节杆菌属杆菌目富集,对照组菌群中乳酸杆科及乳酸杆属富集。在原分组情况下,与对照组比较,观察组和阳性组ADP-heptose/ALPK1 mRNA表达增加(P<0.05);与阳性组比较,观察组ADP-heptose/ALPK1 mRNA表达增加(P<0.05)。根据乳酸杆菌缺乏阳性分组,与对照组比较,观察组和阳性组ADP-heptose/ALPK1 mRNA表达增加(P<0.05);与阳性组比较,观察组ADP-heptose/ALPK1 mRNA表达增加(P<0.05)。根据乳酸杆菌缺乏和微生物群密度阳性分组,与对照组比较,观察组和阳性组ADP-heptose/ALPK1 mRNA表达增加(P<0.05);与阳性组比较,观察组ADP-heptose/ALPK1 mRNA表达增加(P<0.05)。根据乳酸杆菌缺乏阳性分组,观察组和阳性组ADP-heptose mRNA在HPV阳性宫颈癌诊断中的曲线下面积(AUC)值为0.920(0.820~1.000),ALPK1 mRNA为0.833(0.701~0.965),均有�
Objective Toexplore the mechanism of vaginal microbiota on HPV infection based on ADP heptose/ALPK1.Methods Collect 84 HPV positive cervical cancer patients(observation group),107 HPV positive patients without cervical cancer(positive group),and 191 healthy women(control group)from January 2020 to October 2023.Collect vaginal secretions from patients,and use Wright Giemsa staining to detect levels of lactic acid bacteria and microbial diversity and density;Place another swab in a 0.9%sodium chloride solution to measure the cleanliness of secretions and the levels of Candida,Trichomonas,and Lactobacillus;The third swab is used to detect the pH value of vaginal secretions.Bioinformatics analysis of vaginal microbiota,qRT PCR detection of ADP heptose/ALPK1 mRNA levels.Results There were statistically significant differences in the alpha diversity indicators of microbial communities among the three groups:Chao1 index(F=32.780,P<0.05),Ace index(F=6.270,P<0.05),Shannon index(F=17.240,P<0.01),and Simpson index(F=9.710,P<0.05).There was a statistically significant difference in the abundance of Burkholderia and Lactobacillus genera between the three groups(P<0.05).The abundance of Burkholderia genera in the observation group and the positive group was higher than that in the control group(χ^(2)=2.18,P=0.03),while the relative abundance of Lactobacillus species was lower than that of the control group(χ^(2)=3.13,P<0.01).LefSe analysis showed that there were differences in the abundance of four characteristic bacterial genera among the observation group,positive group,and control group(LDA value>2.0).Burkholderia and Arthrobacter are enriched in the observation group and positive group,while Lactobacillus and Lactobacillus are enriched in the control group.In the original group,compared with the control group,the expression of ADP heptose/ALPK1 mRNA increased in the observation group and positive group(P<0.05);Compared with the positive group,the observation group showed an increase in ADP heptose/ALPK1 mRNA expression(P
作者
章昉
寿坚
周建英
繆飞龙
施婷婷
ZHANG Fang;SHOU Jian;ZHOU Jianying;MIAO Feilong;SHI Tingting(The Second People's Hospital of Xiaoshan District,Hangzhou,311241,China;Zhejiang Xiaoshan Hospital;Shangyu Maternal and Child Health Hospital)
出处
《中国病原生物学杂志》
CSCD
北大核心
2024年第5期514-519,523,共7页
Journal of Pathogen Biology
基金
浙江省医药卫生科技计划项目(No.2023KY1013)
上虞市科技局课题(No.2020001)。
