摘要
对3个发酵批次的红茶菌发酵液进行宏基因组序列分析,采用数据库进行比对,发现红茶菌发酵液中存在短乳杆菌、植物乳杆菌、食窦魏斯氏菌、发酵乳杆菌、戊糖乳杆菌、融合魏斯氏菌、罗伊氏乳杆菌、柠檬明串珠菌、类植物乳杆、副干酪乳杆菌、乳酸片球菌等具有益生功能的细菌。宏基因组注释到的功能基因共有76512个,其中与代谢过程注释到的功能基因数最多,且这些代谢途径上注释得到的菌株多数是益生菌株,主要参与了与碳水化合物代谢相关的三羧酸循环、柠檬酸循环、磷酸戊糖途径、糖酵解等;与氨基酸代谢相关主要有丙氨酸、天冬氨酸和谷氨酸代谢途径、甘氨酸、丝氨酸和苏氨酸等代谢途径。
The metagenomic sequence analysis of the fermentation broth of three fermentation batches of kombucha was carried out,and the database was used for comparison.It was found that there were mainly Lactobacillus brevis,Lactobacillus plantarum,Weisseria sinusosus,Lactobacillus fermentatum,Lactobacillus pentosus,Weisseria fused,Lactobacillus reuteri,Nebulococci citronicum,Lactobacillus paracasei,Lococci lactate and other bacteria with probiotic function in the fermentation broth of tea fungus.A total of 76512 functional genes were annotated by metagenomes,among which the most functional genes were annotated by metabolic processes.Most of the strains annotated by these metabolic pathways were probiotic strains,which were mainly involved in the tricarboxylic acid cycle,citric acid cycle,pentose phosphate pathway,glycolysis and so on related to carbohydrate metabolism.The metabolic pathways of alanine,aspartate and glutamate,glycine,serine and threonine are mainly related to amino acid metabolism.
作者
赵平
刘庆静
杨光
郭莹莹
邹积宏
ZHAO Ping;LIU Qing-jing;YANG Guang(Heilongjiang Green Food Science Research Institute,Harbin,Heilongjiang 150028;Heilongjiang University,Harbin,Heilongjiang 150008;Quality Supervision and Testing Institute of Heilongjiang Province,Harbin,Heilongjiang 150028)
出处
《安徽农业科学》
CAS
2024年第9期71-78,106,共9页
Journal of Anhui Agricultural Sciences
基金
国家市场监督管理总局技术保障专项项目(2020YJ010)
黑龙江省财政支持项目。
