摘要
本文运用水培法(1000 mg·L^(−1)的硝酸铅溶液)在苗期对37份玉米品种进行铅胁迫处理,分别测定其根系总长度、根表面积、平均直径、根体积、根尖数、分叉数、交叉数、根系鲜重、根系干重和根冠比等性状,利用方差分析、主成分隶属函数分析、聚类分析、相关性分析、回归分析等进行耐铅性评价及分类。结果表明,在铅胁迫条件下玉米根系性状均受到显著影响,除根系平均直径外,其它根系性状均有一定程度的下降。方差分析表明,根干重处理间呈极显著差异,材料间呈显著差异,其余性状材料间和处理间均呈现极显著差异水平。通过主成分分析和聚类分析,将玉米苗期耐铅性分为6类,即2份高耐品种、3份耐品种、5份中等品种、8份低耐品种、14份敏感品种、5份高敏品种。最后利用相关性和回归分析建立了玉米苗期耐铅性模型:D=−0.287+0.168×根总长度+0.148×根表面积+0.087×根体积+0.041×根尖数+0.125×分叉数+0.119×交叉数+0.138×根系鲜重+0.089×根系干重。本研究综合运用多种分析方法对37份玉米品种进行了苗期耐铅性评价,为修复铅污染土壤修复提供了种质资源。
With the hydroponic method(1000 mg·L^(−1) lead nitrate solution),37 maize varieties was treated with lead stress at the seedling stage,and the total root length,root surface area,average diameter,root volume,root tip number,bifurcation number,cross number,root fresh weight,root dry weight and root-shoot ratio were measured.Furthermore,the lead tolerance of 37 maize varieties was evaluated and classified by analysis of variance,principal component membership function analysis,cluster analysis,correlation analysis and regression analysis.The results showed that the root traits of maize were significantly affected under lead stress,other root traits were all decreased to a certain extent except for the average diameter of the root system.The analysis of variance showed that there were extremely significant differences between root dry weight treatments and materials,and there were significant differences between materials and treatments for other traits.According to the results of principal component membership function analysis and cluster analysis,six clusters were obtained for the lead resistance of maize varieties,namely 3 resistant varieties,5 medium varieties,8 lowtolerance varieties,14 susceptible varieties,5 high-allergy varieties.Finally,correlation and regression analysis were used to establish a model of lead tolerance at maize seedling stage:D=−0.287+0.168×total root length+0.148×root surface area+0.087×root volume+0.041×root tip number+0.125×number of forks+0.119×number of crossings+0.138×root fresh weight+0.089×root dry weight.We applied multiple analytical methods to evaluate the lead tolerance of 37 maize varieties and providing germplasm resources for the remediation of lead-contaminated soils.
作者
马兴业
李双丹
张乐
赵永星
时振坤
陈士林
张晓祥
MA Xing-ye;LI Shuang-dan;ZHANG Le;ZHAO Yong-xing;SHI Zhen-kun;CHEN Shi-lin;ZHANG Xiao-xiang(School of Agriculture,Henan Institute of Science and Technology,Henan Collaborative Innovation Center of Modern Biological Breeding,Xinxiang 453003,China)
出处
《山东农业大学学报（自然科学版）》
北大核心
2024年第2期193-201,共9页
Journal of Shandong Agricultural University：Natural Science Edition
基金
国家自然科学基金项目(32301772)
河南省科技攻关项目(222102110050)。
关键词
玉米
耐铅性鉴定
隶属函数
评价方法
Maize
identification of lead resistance
membership functions
evaluation methodology
