摘要
【目的】研究接种不同根际促生菌(PGPR)对白花前胡根际土壤微生态环境及生长状况的影响,为微生物菌肥资源的开发及白花前胡的品质培优提供参考。【方法】采用室温盆栽方式,设9个接种PGPR处理:S1处理接种苏云金芽孢杆菌(Bacillus thuringiensis),S2处理接种解淀粉芽孢杆菌(Paenibacillus amylolyticus),S3处理接种多粘芽孢杆菌(B. polymyxa),S4处理接种阿氏芽孢杆菌(B. aryabhattai)(筛选地四川),S5处理接种阿氏芽孢杆菌(B. aryabhattai)(筛选地云南),S6处理接种蜡状芽孢杆菌(B. cereus),S7处理接种运动芽孢杆菌(B. mycoides),S8处理接种蛋白水解芽孢杆菌(B. proteolyticu),S9处理接种维德曼芽孢杆菌(B. wiedmannii)。以栽培基质灭菌不接种为对照(CK)。对比分析接种不同PGPR后白花前胡根际土壤微生物数量、理化性状、酶活性及植株生长指标的差异,并进行土壤理化性质、微生物数量与生长指标的相关分析。【结果】接种不同PGPR对白花前胡根际土壤微生物数量均有一定影响,其中,细菌数量、真菌数量、放线菌数量、解无机磷菌数量、解有机磷菌数量和解钾菌数量最高值分别较CK增长295.63%、134.32%、48.17%、81.42%、251.70%和81.20%。接种PGPR后,各处理的根际土壤p H均高于CK,根际土壤碱解氮、速效磷、速效钾(S7处理除外)和有机质含量也有不同程度的提升,整体以S6处理的土壤性状指标表现较优。接种PGPR提高了白花前胡根际土壤的蛋白酶、磷酸酶和蔗糖酶活性,整体以S8处理的酶活性指标表现较优。从生长指标来看,与CK相比,接种PGPR后白花前胡的株高和茎粗增加,叶片变长,叶面积增大,多数处理的叶宽和叶柄长度有所增加。相关分析结果表明,根际土壤pH与叶宽呈显著正相关(P<0.05,下同),速效磷含量与茎粗呈极显著正相关(P<0.01,下同),速效钾含量与叶柄长度呈显著正相关,有机质含量与叶长、叶面积呈极�
【Objective】To study the effects of inoculation of different rhizosphere growth-promoting bacteria(PGPR)on the microecological environment and growth status of rhizosphere soil of Peucedanum praeruptorum Dunn,so as to provide a reference for the development of microbial fertilizer resources and the quality improvement of P.praeruptorum.【Method】Nine PGPR inoculations were used to inoculate the plants at room temperature:S1 treatment inoculated with Bacillus thuringiensis,S2 treatment inoculated with Paenibacillus amylolyticus,and S3 treatment inoculated with (B.poly-myxa),S4 treatment inoculated with B.aryabhattai(screening site was Sichuan),S5 treatment inoculated with B.aryab-hattai(screening site Yunnan),S6 treatment inoculated with B.cereus,S7 treatment inoculated with B.mycoides,S8 treatment inoculated with B.proteolyticu,S9 treatment inoculated with B.wiedmannii.The cultivation substrate was steri-lized without inoculation as a control(CK).The differences in the number of microorganisms,physicochemical traits,en-zyme activities and plant growth indexes in the rhizosphere of P.praeruptorum after inoculation with different PGPR were compared and analyzed,and the correlation analysis of soil physicochemical properties,microbial number and growth in-dexes was carried out.【Result】Inoculation with different PGPR had a certain effect on the number of microorganisms in the rhizosphere soil of P.praeruptorum.The number of bacteria,fungi,actinomycetes,inorganic phosphate-solubilizing bacteria,organic phosphate-solubilizing bacteria and potassium-solubilizing bacteria increased by 295.63%,134.32%,48.17%,81.42%,251.70%and 81.20%respectively compared with the CK.After PGPR inoculation,the pH of rhizo-sphere soil was higher than that of CK,and the contents of alkali-hydrolyzable nitrogen,available phosphorus,available potassium(except S7 treatment)and organic matter in rhizosphere soil were also increased to varying degrees,and the overall soil trait indexes of S6 treatment were fine.PGPR inoculation increased
作者
罗娅
况刚
张萌
芮璐
郭冬琴
周浓
LUO Ya;KUANG Gang;ZHANG Meng;RUI Lu;GUO Dong-qin;ZHOU Nong(College of Food and Biological Engineering/Chongqing Engineering Laboratory for Green Cultivation and Deep Pro-cessing of Three Gorges Reservoir Area’s Medicinal Herbs,Chongqing Three Gorges University,Chongqing 404120,China;College of Biological and Chemical Engineering,Chongqing University of Education,Chongqing 400067,China;College of Pharmacy,Dali University,Dali,Yunnan 671000,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2024年第1期75-85,共11页
Journal of Southern Agriculture
基金
重庆市自然科学基金项目(CSTC2021jcyj-msxmX0115)
重庆市教育委员会科学技术研究项目(KJQN202201225)。
关键词
根际促生菌
白花前胡
微生物数量
土壤酶活性
土壤养分
plant growth-promoting rhizobacteria
Peucedanum praeruptorum Dunn
microbial quantity
soil enzymatic activity
soil nutrient
