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氮自掺杂碳量子点的合成及其在细胞成像中的应用

Synthesis of nitrogen self-doped carbon quantum dots and their application in cell imaging
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摘要 以特色农产品废弃物马蹄皮为原料,通过水热法合成了氮掺杂碳量子点(N-CQDs),对温度及反应时间等合成条件进行了优化。在最佳合成条件下,得到量子产率为22%的N-CQDs,对其结构、形貌、组成、荧光及电化学性能进行分析。实验结果表明,N-CQDs稳定性良好,表现出更优异的荧光性能和电化学性能,且具有良好的生物相容性和细胞膜穿透性,可用于细胞内多色细胞成像。本研究为促进NCQDs的绿色合成及其在生物医学领域的应用提供了新的思路。 Nitrogen-doped carbon quantum dots(N-CQDs)were synthesized by hydrothermal method using horseshoe skin as raw material.The synthesis conditions of temperature and reaction time were optimized.N-CQDs with a quantum yield of 22%were obtained under the optimal synthesis conditions.The structure,morphology,composition,fluorescence and electrochemical properties of the synthesized N-CQDs were analyzed.The experimental results showed that N-CQDs were stable and exhibited better fluorescence and electrochemical properties,and had good biocompatibility and cell membrane penetration,which could be used for intracellular multicolor cell imaging.This study provides a new way to promote the green synthesis and application of N-CQDs in the biomedical field.
作者 瞿金为 杨淑琼 陈盛余 胡月芳 QU Jinwei;YANG Shuqiong;CHEN Shengyu;HU Yuefang(Guangxi Key Laboratory of Calcium Carbonate Resources Comprehensive Utilization,College of Materials and Chemical Engineering,Hezhou University,Hezhou 542899,China;Key Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi,College Chemistry and Environment Engineering,Baise University,Baise 533000,China)
机构地区 贺州学院 百色学院
出处 《分析试验室》 EI CAS CSCD 北大核心 2024年第4期564-571,共8页 Chinese Journal of Analysis Laboratory
基金 广西自然科学基金面上项目(2022GXNSFAA035469,2021GXNSFAA220113,2017GXNSFAA198274) 广西科技基地和人才专项资助项目(桂科AD23026079,桂科AD23023008) 贺州学院博士和教授科研启动基金项目(HZUBS202105,HZUJS202204)资助。
关键词 氮自掺杂碳量子点 水热法 光学性能 电化学性能 细胞成像 nitrogen-doped carbon quantum dots hydrothermal synthesis method optical performance electro-chemical properties cell imaging
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