摘要
以新疆、内蒙古、山西、陕西、甘肃6个产地的沙棘种子为试材,采用高通量测序及生物信息学分析方法,基于16S rRNA和ITS2序列,研究了沙棘种子内生细菌和真菌的菌群结构与多样性,以期为后续研究沙棘种子内生菌的生物活性,探寻沙棘内生功能菌群提供参考依据。结果表明:沙棘种子内生菌组成较为单一,内生细菌主要为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)等,内生真菌为子囊菌门(Ascomycota)、被孢霉门(Mortierellomycota)和担子菌门(Basidiomycota);Alpha物种多样性分析结果表明,陕西志丹与新疆阿勒泰样品内生细菌的Shannon指数与Simpson指数存在显著差异;山西吕梁与陕西志丹样品内生真菌的Shannon指数和Chao1指数达到显著差异;NMDS分析显示不同样品的内生菌组成存在一定相似性;KEGG分析内生细菌共获得6个一级代谢系统和26个二级代谢通路。综上,不同产地沙棘种子内生菌多样性与群落组成存在差异性,这可能与其栖息地的环境因子密切相关。
In order to provide reference for the subsequent study of the biological activity of endophytes and explore the endophytic functional microbiota in Hippophae rhamnoides seeds,six samples were collected from Xinjiang,Inner Mongolia,Shanxi,Shaanxi and Gansu,the structure and diversity of endophytic bacteria and fungi were studied by high-throughput sequencing and bioinformatics analysis methods based on 16S rRNA and ITS2 sequence.The results showed that the composition of endophytes in H.rhamnoides seeds was relatively simple.The endophytic bacteria were mainly Proteobacteria,Firmicutes,Actinobacteria;the endophytic fungi were Ascomycete,Mortierellomycota and Basidiomycota.Alpha diversity analysis showed that the Shannon and Simpson indices of endophytic bacteria in Zhidan,Shaanxi and Altay,Xinjiang samples were significantly different.Meanwhile,the Shannon and Chao1 indices of endophytic fungi were significantly different between seed samples from Lyuliang,Shanxi and Zhidan,Shaanxi.Non-metric multidimensional scaling analysis showed certain similarity on the composition of endophytes in different seed samples.Kyoto Encyclopedia of Genes and Genomes analysis generated six primary metabolic systems and 26 secondary metabolic pathways in the endophytic bacteria.Accordingly,the diversity and community composition of endophytes in H.rhamnoides seeds from different regions were different,which may be closely related to their growing environments.
作者
李铂
高晓敏
王楠
黄文静
张永生
宋忠兴
LI Bo;GAO Xiaomin;WANG Nan;HUANG Wenjing;ZHANG Yongsheng;SONG Zhongxing(Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Shaanxi University of Chinese Medicine/State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Xianyang,Shaanxi 712083;Inner Mongolia Haitian Pharmaceutical Co.Ltd.,Tongliao,Inner Mongolia 028000)
出处
《北方园艺》
CAS
北大核心
2024年第8期94-101,共8页
Northern Horticulture
基金
内蒙古自治区2019年度重大科技专项资助项目(2019ZD005)
国家中药材产业技术体系建设专项资助项目(CARS-21)
陕西省高校青年科技创新团队资助项目(陕教[2019]90号)。
关键词
沙棘
种子
内生菌
高通量测序
多样性
Hippophae rhamnoides
seeds
endophytes
high-throughput sequencing
diversity
