摘要
目的观察转录因子TEAD1在小鼠急性心肌梗死后心肌成纤维细胞中的表达情况,为探究其作用机制提供理论基础。方法取40只SPF级雄性C57BL/6小鼠随机分为急性心肌梗死组(AMI组)和假手术组(Sham组),术后以普通饲料喂养至1、3、7、14 d处死并取材。本研究以实时心电图变化、TTC染色判断造模是否成功,使用蛋白印迹法检测心脏中TEAD1的表达情况,使用TEAD1免疫荧光和麦胚凝集素(WGA)染色双标法检测TEAD1在心肌组织中的表达情况,使用免疫荧光双标法检测TEAD1在心肌成纤维细胞中的表达情况。结果与Sham相比,AMI组第3天和第7天TEAD1在心脏中表达明显增加(P<0.05),AMI组各天数梗死交界区TEAD1表达量明显增加(P<0.05),呈现先增后减的趋势,且第3天表达量最多,各相邻天数TEAD1的表达量之间差异均具有统计学意义(P<0.05);免疫荧光双标结果显示转录因子TEAD1在急性心肌梗死后心肌组织的心肌成纤维细胞中呈现高表达,且相较于Sham组,AMI组表达TEAD1的心肌成纤维细胞数量在急性心肌梗死后明显增加(P<0.05),以第7天和第14天较多,并主要在梗死交界区表达。结论TEAD1在急性心肌梗死后心肌组织中梗死交界区表达明显增加,且在心肌成纤维细胞中呈现高表达。
Objective To observe the expression of transcription factor(TEAD1)in myocardial fibroblasts after acute myocardial infarction in mice,and to provide a theoretical basis for exploring its mechanism of action.Methods Forty SPF grade male C57BL/6 mouse were selected and randomly divided into acute myocardial infarction group(AMI group)and sham-operation group(Sham group).After surgery,they were fed with ordinary feed until day 1,3,7,and 14,and then sacrificed and sampled.The real-time electrocardiogram changes and TTC staining were used to judge whether the model succeed.The expression of TEAD1 in the heart was detected by Western blotting.The expression of TEAD1 in myocardial tissue was detected by double labeling of immunofluorescence and wheat germ agglutinin(WGA)staining.The expression of TEAD1 in myocardial fibroblasts was detected by double labeling of immunofluorescence.Results Compared with Sham,the expression of TEAD1 in the heart increased significantly on the 3rd and 7th day in AMI group(P<0.05).The expression of TEAD1 in the infarct border zone increased significantly on each days in AMI group(P<0.05),showing a trend of first increasing and then decreasing,and the expression level was highest on the 3rd day.There was a statistically significant difference in the expression level of TEAD1 between adjacent days(P<0.05).The results of double labeling of immunofluorescence showed that TEAD1 was highly expressed in myocardial fibroblasts in myocardial tissue after acute myocardial infarction.Compared with Sham group,the quantity of cell expression TEAD1 in myocardial fibroblasts increased significantly after acute myocardial infarction(P<0.05),mainly expressed in the infarct border zone on 7th and 14th day.Conclusion The expression of TEAD1 in the infarct border zone of myocardial tissue increased significantly after acute myocardial infarction,and it is highly expressed in myocardial fibroblasts.
作者
张驰
李礼
燕茹
裴建升
张萌
贾绍斌
ZHANG Chi;LI Li;YAN Ru;PEI Jiansheng;ZHANG Meng;JIA Shaobin(School of Clinical Medicine,Ningxia Medical University,Yinchuan 750004,China;Heart Center,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Ningxia Medical University,Yinchuan 750004,China)
出处
《宁夏医学杂志》
CAS
2024年第4期277-280,F0002,共5页
Ningxia Medical Journal
基金
宁夏自然科学基金项目(2022AAC03479)
宁夏医科大学校级基金项目(XM2021008)。
