摘要
以荷叶离褶伞菌多糖LDS-1、甘露聚糖肽和空白对照作用下的小鼠S180肿瘤细胞为研究材料,运用RNA-Seq测序技术进行生物信息学分析,结果显示LDS-1组、甘露聚糖肽组和空白对照组分别获得8.27、 8.35和8.97 G的分析数据.高表达基因(FPKM>2 200)分析显示LDS-1主要通过下调Rplp1基因调控细胞分裂,将S180细胞停滞在G1期以抑制其分裂.差异基因分析结果显示LDS-1主要通过上调Alb、 Timp4、 Apoa1基因调节蛋白表达或者细胞黏连性,抑制S180肿瘤细胞迁移.KEGG(京都基因与基因组百科全书)通路富集结果显示, LDS-1抑制S180肿瘤生长的关键基因主要富集在过氧化物酶体增殖物激活受体(PPAR)信号通路中,上游Fabp3基因下调,激活下游Apoa1、Slc27a2和Adipoq等多个基因上调,调控S180细胞的脂质代谢和胆固醇代谢. LDS-1能够通过调控高表达关键基因Rplp1,差异表达关键基因Alb、 Timp4、 Apoa1,以及PPAR信号通路抑制S180肿瘤细胞的增殖分裂、能量获取和迁移侵袭.
The mouse S180 tumor cells treated with polysaccharide LDS-1,mannatide and blank control were used as research materials.The data were sequenced via RNA-Seq and analyzed by the bioinformatics method.The results showed that 8.27,8.35 and 8.97 G of the analytical data were obtained in the RNALDS-1,mannan peptide and blank control group respectively.Analysis of highly expressed genes(FPKM>2200)showed that LDS-1 regulated cell division mainly by down-regulating Rplp1 gene and inhibited the division of S180 cells in G1 phase.A differential gene analysis showed that LDS-1 inhibited the migration of S180 tumor cells mainly by up-regulating the expression of Alb,Timp4 and Apoa1 gene regulatory proteins or cell adhesion.Kyoto encyclopedia of genes and genomes pathway enrichment results showed that the key genes of LDS-1 inhibiting S180 tumor growth were mainly enriched in the peroxisome proliferator activated receptor(PPAR)signaling pathway.Fabp3 gene in the upstream of this pathway was down-regulated and several genes such as Apoa1,Slc27a2 and Adipoq were up-regulated in the downstream,which regulated lipid metabolism and cholesterol metabolism of S180 cells.It was found that LDS-1 could inhibit the proliferation,division,energy acquisition,migration and invasion of S180 tumor cells through regulating the high expression of key genes Rplp1,differential expression of key genes Alb,Timp4 and Apoa1,and the PPAR signaling pathway.
作者
王艺学
刘影
赵倪慧
卢睿加
侯怡铃
丁祥
WANG Yi-xue;LIU Ying;ZHAO Ni-hui;LU Rui-jia;HOU Yi-ling;DING Xiang(College of Environmental Science and Engineering,Nanchong Key Laboratory of Eco-environmental Protection and Pollution Control in Jialing River Basin,China West Normal University,Nanchong 637009,Sichuan,China;College of Life Science,Key Laboratory of Southwest China Wildlife Resources Conservation,the Ministry of Education,China West Normal University,Nanchong 637009,Sichuan,China)
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
2024年第2期276-284,共9页
Journal of Lanzhou University(Natural Sciences)
基金
四川省科技厅应用基础面上项目(2022NSFSC0107)
四川省科技厅农业科技成果转化资金项目(2022NZZJ0003)
四川省转移支付科技成果转移转化示范项目(22ZYZFSF0009,23ZHSF0082)。
