摘要
[目的]研究一氧化碳(CO)和硫化氢(H_(2)S)调控作物幼苗抗盐能力的机理,为缓解盐渍土中NaCl对作物的胁迫提供理论和技术。[方法]以加工番茄品系KT-7为试验材料,采用无土栽培方法进行CO和H_(2)S叶面喷施试验。CO供体为氯化血红素溶液(hemin 15μmol/L),H_(2)S供体为硫氢化钠溶液(NaHS,50μmol/L),并因处理需要使用了H_(2)S清除剂次牛磺酸溶液(HT,100μmol/L)和血红素加氧酶抑制剂锌原卟啉溶液(ZnPPⅨ,25μmol/L)。试验共设6个处理,除对照根部浇灌营养液,叶面喷施清水外(CK_(0)),其余5个处理均根部浇灌200 mmol/L NaCl溶液,叶面分别喷施清水(CK_(1))、CO、H_(2)S、CO+HT、H_(2)S+ZnPPⅨ。幼苗长至四叶一心时,连续6天进行NaCl浇灌和叶面喷施处理,然后测定幼苗形态学指标,用Li-6800便携式光合测定仪测定光合参数,用Imaging-PAM荧光成像系统测定叶绿素荧光系数,植物效率仪测定叶绿素荧光动力学曲线(OJIP曲线)。[结果]胁迫6天后,加工番茄幼苗的各项形态学参数、净光合速率(P_(n))、实际光化学效率[Y(Ⅱ)]和光系统Ⅱ(PSⅡ)性能指标[PI_(ABS)和PI_((CSM))]显著下降,OJIP曲线产生形变,并出现K点。CO和H_(2)S处理均有效提高了加工番茄幼苗的光合色素含量、光合参数、PSⅡ的综合性能和受体侧的电子传递效率;提升了OJIP曲线中的J点、I点、P点,降低了ΔK,从而缓解了OJIP曲线的形变;CO处理的P_(n)、表观CO^(2)利用率(CUEapp)、Y(Ⅱ)和电子传递速率(ETR)分别较CK_(1)上升了75.63%、100%、60.00%和57.72%,H_(2)S处理分别上升了65.95%、100%、55.00%和52.68%,CO和H_(2)S处理均降低了非光化学淬灭和热耗散的比例,减少了PSⅡ供体侧放氧复合体(OEC)的损伤。CO+HT处理缓解NaCl胁迫的效果和CO处理相似,而H_(2)S+ZnPPⅨ处理则不能缓解NaCl胁迫,其光合色素含量、P_(n)、F_(v)/F_(m)、Y(Ⅱ)、qL、ETR、PI_(ABS)和PI_((CSM))较H_(2)S处理显著下降,热耗散比率则较H_(2)S
[Objectives]We studied the mechanism of carbon monoxide(CO)and hydrogen sulfide(H_(2)S)on alleviating NaCl stress to crop seedlings,to provide a theoretical basis and technology for efficient crop production in the secondary salinized soil.[Methods]A soilless cultivation experiment was carried out using a processing tomato strain KT-7 as the test material.The donor for carbon monoxide(CO)was hemin solution(15μmol/L),and for H_(2)S was hypotaurine solution(50μmol/L).In addition,zinc protoporphyrin solution(25μmol/L)was used for heme oxygenase inhibitor,and H_(2)S scavenger hypotaurine solution(HT,100μmol/L)was used as the requirement of the treatments.There were 6 treatments in the experiment,except for CK0(irrigating normal nutrient solution and foliar spraying clean water),the other five treatments were all irrigated with a nutrient solution containing 200 mmol/L NaCl,and foliar sprayed clean water(CK_(1)),CO,CO+HT,H_(2)S,and H_(2)S+ZnPPⅨ,respectively.When the seedlings were at the stage of 4 leaves-1-sprout,NaCl stress and foliar spraying were carried out for consecutive six days then the morphological indicators were investigated,Li-6800 portable photosynthetic system was used to measure the photosynthetic parameters.The Imaging-PAM modulated fluorescence imaging system was used to determine the fluorescence parameters of chlorophyll,and the plant efficiency meter was used to measure the leaf green fluorescence kinetic curve(OJIP).[Results]After 6 days of NaCl stress,the net photosynthetic rate(P_(n)),actual photochemical efficiency[Y(Ⅱ)]and PSⅡperformance indicators(P_(IABS)and PI_((CSM)))of the seedling leaves decreased significantly,the OJIP curve was deformed,and the K point appeared.Both CO and H_(2)S treatments improved the photosynthetic pigment content,photosynthetic parameters,comprehensive performance of PSⅡand electron transfer efficiency on the receptor side,revised the deformation of the OJIP curve by raised the J point,I point,and P point,and fallingΔK.Compared with CK,the Pn,apparen
作者
骆霞
滕元旭
崔辉梅
LUO Xia;TENG Yuan-xu;CUI Hui-mei(College of Agriculture,Shihezi University/Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization,Shihezi,Xinjiang 832003,China)
出处
《植物营养与肥料学报》
CAS
CSCD
北大核心
2024年第3期538-549,共12页
Journal of Plant Nutrition and Fertilizers
基金
国家自然科学基金项目(31860551)
兵团财政科技计划项目(2021AB018)。
关键词
一氧化碳
硫化氢
加工番茄
NACL胁迫
叶绿素荧光
carbon monoxide
hydrogen sulfide
processing tomato
NaCl stress
chlorophyll fluorescence
