摘要
以甘草-石膏(gancao-shigao,GC-SG)药对为研究对象,运用中药超分子化学探讨中医临床应用植物药GC配伍难溶性矿物药SG的科学内涵。①观察GC、SG单煎及共煎液的丁达尔效应、微观形貌并测定其粒径、出膏量和电导率,运用等温滴定量热法(isothermal titration calorimetry,ITC)和红外吸收光谱法(infrared absorption spectroscopy,IR)检测GC-SG之间的相互作用。②以等SG质量的可溶性钙盐氯化钙(CaCl_(2))代替SG与GC共煎探究钙盐含量对水煎剂的影响,结合丁达尔效应、微观形貌、出膏量及电导率进行表征,并运用ITC和IR技术检测二者相互作用;对比研究GC-SG、GC-CaCl_(2)共煎液zeta电位、紫外-可见分光光度法(ultraviolet-visible spectrophotometry,UV-vis)光谱差异,并结合电感耦合等离子体发射光谱仪(inductively coupled plasma optical emission spectrometer,ICP-OES)和高效液相色谱仪(high performance liquid chromatography,HPLC)检测共煎液中的无机和有机成分含量差异。结果表明:①与单煎液相比,GC-SG共煎液有更明显的丁达尔效应,且电镜下呈均匀球形纳米颗粒,出膏量、电导率等物理表征结果显示共煎促进彼此成分溶出;ITC和IR结果表明GC-SG之间存在较强相互作用,初步表明GC-SG共煎促使形成均匀、稳定的中药超分子体系。②以可溶性钙盐代替难溶性SG与GC共煎,出现较单煎液强但散光的光路,zeta电位降低,形成大量聚沉物;出膏量和电导率结果显示共煎较单煎成分溶出减少;ITC、UV-vis和IR结果表明GC与SG、Ca^(2+)之间均有相互作用,GC-Ca^(2+)聚沉物的形成表明大量可溶性钙盐会破坏中药超分子的稳定性;ICP-OES和HPLC结果显示,GC-Ca^(2+)中的甘草酸(glycyrrhizic acid,GA)成分明显低于GC-SG,这与随着Ca^(2+)浓度升高形成大量聚沉物,降低GA等有效成分溶出有关。本研究表明GC-SG配伍可形成均匀、稳定的中药超分子体系,以SG中主要成分钙盐为切入点,过�
Licorice-gypsum(gancao-shigao,GC-SG)drug pair was used as the research object,using supramolecular chemistry to explore the scientific connotation of combining herbal medicine GC with insoluble mineral medicine SG in clinical application of traditional Chinese medicine.①The Tyndall effect,microscopic morphology and particle size of the single and co-decocted of GC and SG were observed,the paste content and conductivity were determined,and the interaction between GC and SG was detected by isothermal titration calorimetry(ITC)and infrared absorption spectroscopy(IR).②Calcium chloride(CaCl_(2)),a soluble calcium salt of equal gypsum quality,was used instead of SG with GC for co-decocting to explore the effect of calcium salt content on the water decocting,and the characteristics were combined with the Tyndall effect,microscopic morphology,paste content and conductivity.ITC and IR techniques were used to detect the interaction between the two,and the interaction between them was detected by ITC and IR.The zeta potential and ultraviolet-visible spectrophotometry(UV-vis)of GC-SG and GC-CaCl_(2) co-decoction were compared,and the inorganic and organic components in the co-decoction were detected by inductively coupled plasma optical emission spectrometer(ICP-OES)and high performance liquid chromatography(HPLC).The results showed:①Compared with the liquid phase of single decoction,GC-SG co-decoction had more obvious Tyndall effect,and showed uniform spherical nanoparticles under electron microscope.Physical characterization results such as paste content and conductivity showed that co-decoction promoted the dissolution of each other's components;ITC and IR results showed that there was strong interaction between GC and SG,which preliminatively indicated that GC and SG co-decoction promoted the formation of uniform and stable supramolecular system of traditional Chinese medicine.②When soluble calcium salt was used to substitute insoluble SG with GC for co-decocting,a stronger but astigmatic light path appeared tha
作者
张瑶芝
皮雯敏
吴林颖
杨璐平
姚舒畅
张翔
黄雪梅
王鹏龙
ZHANG Yao-zhi;PI Wen-min;WU Lin-ying;YANG Lu-ping;YAO Shu-chang;ZHANG Xiang;HUANG Xue-mei;WANG Peng-long(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《药学学报》
CAS
CSCD
北大核心
2024年第4期1048-1056,共9页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(82274072)
中央高校基金科研业务项目(2023-JYB-JBZD-049,2022-XJ-KYQD-008)
国家级高层次青年人才支持计划.
关键词
甘草
石膏
难溶性矿物药
中药超分子
可溶性钙盐
配伍
licorice
gypsum
insoluble mineral medicine
Chinese medicine supramolecule
soluble calcium salt
compatibility
