摘要
为了探讨促肾上腺皮质激素释放激素(CRH)在小鼠妊娠期间血浆中的浓度变化及其与小鼠妊娠持续时间的关系,选取自然交配的见栓雌鼠,自见栓当天开始,通过面颊采血分离、收集血浆,采用酶联免疫吸附试验测定CRH含量。通过对孕鼠妊娠期间、分娩当天及分娩后24 h血浆CRH水平的分析,发现正常妊娠孕鼠血浆中CRH水平在妊娠早期即升高,妊娠13.5 d起血浆CRH水平[(21.6±3.90)pg/mL]呈进行性升高,妊娠晚期升高显著,至分娩时达最高水平,分娩后24 h CRH基本恢复至正常值;早产孕鼠13.5 d时血浆含量高于正常妊娠的小鼠,而难产孕鼠的CRH则明显低于正常妊娠小鼠;孕鼠13.5 d血浆中CRH的含量与妊娠持续时间呈负相关。说明小鼠血浆中CRH水平可作为判定妊娠持续时间的一个标志,用于预测孕鼠的分娩时间,为小鼠的剖宫产生物净化提供理论基础。
In order to investigate the changes of plasma concentrations of corticotrophin-releasing hormone(CRH)in pregnancy and its relationship with the duration of pregnancy in mice,the female mice were selected for mating naturally,from the day of vaginal suppositories was seen,the plasma was separated and collected,and CRH content was determined by enzyme-linked immunosorbent assay.By analyzing the plasma CRH levels of pregnant mice during pregnancy,delivery day and 24 hours after delivery,it was found that the CRH levels in the plasma of normal pregnant mice increased in the early stages of pregnancy.The plasma CRH level[(21.6±3.90)pg/mL]showed a progressive increase starting from 13.5 days of pregnancy,with a significant increase in late pregnancy and reached its highest level at delivery.CRH content basically returned to normal values 24 hours after delivery.The CRH content of preterm pregnant mice at 13.5 days was higher than that of normal pregnant mice,while the CRH content of dystocia pregnancy mice was significantly lower than that of normal pregnancy mice.The CRH content in the plasma of pregnant mice at 13.5 days was negatively correlated with the duration of pregnancy.These results suggest that the levels of CRH in mouse plasma can be used as a marker to determine the duration of pregnancy,to predict the delivery time of pregnant mice,and providing a theoretical basis for biological purification of cesarean section in mice.
作者
张曼
彭丽娜
王朝霞
ZHANG Man;PENG Li-na;WANG Zhao-xia(Laboratory Animal Center of Shanghai Jiao Tong University,Shanghai,200240,China)
出处
《动物医学进展》
北大核心
2024年第5期46-50,共5页
Progress In Veterinary Medicine
基金
上海市兽医生物技术重点实验室开放课题项目(SHKLAB202009)。
关键词
促肾上腺皮质激素释放激素
分娩
妊娠
生物净化
corticotrophin-releasing hormone(CRH)
delivery
pregnancy
biological purification
