摘要
本试验旨在研究混合比例、菌剂和压实密度对玉米秸秆和花生秧混合青贮发酵品质和营养价值的影响。试验分为2部分,试验1研究混合比例和菌剂对玉米秸秆和花生秧混合青贮发酵品质和营养价值的影响。将玉米秸秆和花生秧按5个混合比例(4∶0、3∶1、2∶2、1∶3、0∶4)进行混合,每个比例下设4个组,分别为对照组(CON组,喷洒无菌生理盐水)、植物乳杆菌组(LP组,喷洒1×10^(6)CFU/g植物乳杆菌)、粪肠球菌组(EF组,喷洒1×10^(6)CFU/g粪肠球菌)、植物乳杆菌+粪肠球菌组(LP+EF组,喷洒5×10^(5)CFU/g植物乳杆菌+5×10^(5)CFU/g粪肠球菌),每组3个重复,厌氧发酵60 d。试验2研究压实密度和菌剂对玉米秸秆和花生秧混合青贮发酵品质和营养价值的影响。将玉米秸秆和花生秧按3∶1比例混合后,按不同压实密度(300、450、600 kg/m^(3))进行发酵,每个压实密度下设4个组(同试验1),每组3个重复,厌氧发酵60 d。试验1结果表明:1)随着花生秧比例的提高,有机物、中性洗涤纤维和水溶性碳水化合物含量显著降低(P<0.05),粗蛋白质含量显著升高(P<0.05)。与对照组相比,EF和LP+EF组的中性洗涤纤维含量显著降低(P<0.05),EF组的氨态氮含量显著降低(P<0.05)。2)混合比例对乳酸菌和霉菌数量无显著影响(P>0.05)。与对照组相比,LP、EF和LP+EF组的乳酸菌数量显著升高(P<0.05),LP+EF组的霉菌数量显著降低(P<0.05)。3)混合比例为2∶2组的玉米赤霉烯酮含量显著高于其他各组(P<0.05),混合比例为3∶1和2∶2组的赭曲霉毒素含量显著低于混合比例为4∶0组(P<0.05)。与对照组相比,LP+EF组的玉米赤霉烯酮含量显著降低(P<0.05)。4)混合比例为2∶2组的pH显著高于其他各组(P<0.05),混合比例为3∶1组的乳酸含量显著高于混合比例为2∶2组(P<0.05),混合比例为3∶1组的乙酸含量显著低于其他各组(P<0.05),混合比例为3∶1组的丁酸含量显著低于混合比例为
This experiment was conducted to study the effects of mixing ratio,microbial preparation and packing density on fermentation quality and nutritional value of mixed silage of corn straw and peanut vine.This study contained two experiments.Experiment 1 studied the effects of mixing ratio and microbial preparation on fermentation quality and nutritional value of mixed silage of corn straw and peanut vine.The corn straw and peanut vine were mixed based on 5 mixing ratios(4∶0,3∶1,2∶2,1∶3 and 0∶4),with 4 groups in each ratio,involving control group(CON group,spraying sterile physiological saline),Lactobacillus plantarum group(LP group,spraying 1×10^(6) CFU/g Lactobacillus plantarum),Enterococcus faecalis group(EF group,spraying 1×10^(6) CFU/g Enterococcus faecalis)and Lactobacillus plantarum+Enterococcus faecalis group(LP+EF group,spraying 5×10^(5) CFU/g Lactobacillus plantarum+5×10^(5) CFU/g Enterococcus faecalis).Each group contained 3 replicates,and anaerobic fermentation for 60 days.Experiment 2 studied the effects of packing density and microbial preparation on fermentation quality and nutritional value of mixed silage of corn straw and peanut vine.The corn straw and peanut vine were mixed at a ratio of 3∶1,and fermented with different packing densities(300,450 and 600 kg/m^(3)),with 4 groups in each density(same as experiment 1).Each group contained 3 replicates,and anaerobic fermentation for 60 days.The results of experiment 1 showed as follows:1)with the increased of peanut vine proportion,the organic matter,neutral detergent fiber and water-soluble carbohydrate contents were significantly decreased(P<0.05),while the crude protein content was significantly increased(P<0.05).Compared with the control group,the neutral detergent fiber content in EF and LP+EF groups was significantly decreased(P<0.05),and the ammonia nitrogen content in EF group was significantly decreased(P<0.05).2)The mixing ratio had no significant effects on the lactic acid bacteria and mold numbers(P>0.05).Compared with the co
作者
张相伦
张正
李俊玲
杨赵军
盛清凯
刘晓牧
赵国朵
赵红波
ZHANG Xianglun;ZHANG Zheng;LI Junling;YANG Zhaojun;SHENG Qingkai;LIU Xiaomu;ZHAO Guoduo;ZHAO Hongbo(Key Laboratory of Livestock and Poultry Multi-Omics of The Ministry of Agriculture and Rural Affairs,Shandong Key Laboratory of Animal Disease Control and Breeding,Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Ji’nan 250100,China;Institute of Crop Germplasm Resources,Shandong Academy of Agricultural Sciences,Ji’nan 250100,China;Shandong Animal Product Quality and Safety Center,Ji’nan 250100,China;Key Laboratory of Ruminant Nutrition and Feed of Ji’nan,Shandong Meishida Agriculture and Husbandry Technology Limited Company,Ji’nan 251400,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2024年第4期2633-2647,共15页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
山东省现代农业产业技术体系牛产业创新团队(SDAIT-09-04)
山东省科技型中小企业创新能力提升工程(2022TSGC2071,2022TSGC1288)
山东省农业科学院农业科技创新工程(CXGC2023A23)
山东省农业重大技术协同推广计划(SDNYXTTG-2023-26、SDNYXTTG-2023-12)。
关键词
玉米秸秆
花生秧
混合青贮
菌剂
压实密度
corn straw
peanut vine
mixed silage
microbial preparation
packing density
