摘要
本研究旨在探讨全反式视黄酸(ATRA)对昆明小鼠卵泡发育、繁殖性能和生殖激素水平的影响。试验选取7周龄雌性昆明小鼠24只,适应性饲养5~7 d后,随机分为4组,每组6个重复,每个重复1只,分别为对照组(灭菌蒸馏水)、低剂量组(0.1 mg/kg ATRA)、中剂量组(1.0 mg/kg ATRA)和高剂量组(10.0 mg/kg ATRA),连续灌胃14 d。各组分别取6只昆明小鼠采样,测定血清雌激素(E_(2))、孕酮(P_(4))和促卵泡生成素(FSH)水平,同时分离卵巢并计算卵巢系数,制作卵巢组织石蜡切片,并进行卵泡计数。选择未发情的昆明小鼠64只,随机分为4组,每组16个重复,每个重复1只。剂量同上。连续灌胃ATRA 1个发情周期(5 d)。将发情小鼠各组取6只采样,测定血清激素水平,其余10只与雄性小鼠合笼,妊娠期间每周称量1次体重,分娩之后统计窝产仔数、出生窝重与出生个体重。结果表明:1)昆明小鼠连续灌胃ATRA 14 d,与对照组相比,ATRA对小鼠的体重与卵巢系数均没有显著影响(P>0.05)。2)与对照组相比,高剂量组初级卵泡的数量显著提高(P<0.05),而闭锁卵泡数量显著低于对照组和低剂量组(P<0.05)。3)连续灌胃ATRA 1个发情周期(5 d),各ATRA组昆明小鼠妊娠期间体重没有显著变化(P>0.05)。4)高剂量组昆明小鼠窝产仔数显著高于对照组(P<0.05),中剂量组和高剂量组的昆明小鼠出生窝重显著高于对照组(P<0.05),试验组与对照组小鼠相比出生个体重没有显著差异(P>0.05)。5)昆明小鼠连续灌胃ATRA 5 d,高剂量组血清中E_(2)、P_(4)和FSH水平均显著高于对照组(P<0.05),且高剂量组的E_(2)水平显著高于低剂量组和中剂量组(P<0.05),同时中剂量组的P_(4)水平也显著高于对照组和低剂量组(P<0.05);昆明小鼠连续灌胃ATRA 14 d,高剂量组血清中E_(2)、P_(4)和FSH水平均显著高于对照组(P<0.05),同时高剂量组P_(4)水平也显著高于低剂量组(P<0.05)。综上所述,ATRA可促进昆明小鼠E_(
The aim of this experiment was to investigate the effects of all-trans retinoic acid(ATRA)on follicular development,reproductive performance and reproductive hormone levels in Kunming mice.Twenty-four female Kunming mice aged 7 weeks were selected.After 5 to 7 days of adaptive feeding,they were randomly divided into 4 groups with 6 replicates per group and 1 mouse per replicate.The mice were divided into control group(sterilized distilled water),low-dose group(0.1 mg/kg ATRA),medium-dose group(1.0 mg/kg ATRA)and high-dose group(10.0 mg/kg ATRA).ATRA was given gavage for 14 d.Six Kunming mice were sampled from each group,and serum estrogen(E_(2)),progesterone(P_(4)),and follicle stimulating hormone(FSH)levels were measured.Their ovaries were isolated and ovarian coefficients were calculated,paraffin sections of ovarian tissue were prepared and the number of the different levels of follicle were counted.Sixty-four proestrus Kunming mice were randomly divided into 4 groups with 16 replicates per group and 1 mouse per replicate.Gavage ATRA continuously for 1 estrous cycle(5 d)and the dosages of ATRA were as above.Six mice were sampled from each group of estrous mice and their serum hormone levels were determined.The remaining 10 mice were cohabited with male mice and weighed once a week during gestation.The litter size,litter birth weight and individual birth weight were counted after the mice delivery.The results showed as follows:1)there was no significant effects of ATRA on body weight and ovarian coefficient of Kunming mice by continuous gavaging ATRA for 14 days compared with the control group(P>0.05).2)The number of primary follicles in the high-dose group was significantly higher than that in the control group(P<0.05),and the number of atretic follicles was significantly lower than that in the control group and the low-dose group in the high-dose group(P<0.05).3)Continuous gavage of ATRA for one oestrus cycle(5 days)did not result in significant changes in body weight during pregnancy in all ATRA groups of Kun
作者
赵明
李琳
王桦彪
陈秀文
王水莲
张虹亮
ZHAO Ming;LI Lin;WANG Huabiao;CHEN Xiuwen;WANG Shuilian;ZHANG Hongliang(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2024年第4期2623-2632,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金青年基金项目(31802151)
中国博士后科学基金面上项目(2018M642981)
湖南省自然科学基金青年基金项目(2019JJ50257)。
关键词
全反式视黄酸
小鼠
卵泡发育
繁殖性能
生殖激素
all-trans retinoic acid
mice
follicular development
reproductive performance
reproductive hormones
