摘要
目的:通过观察电针(EA)对大鼠海马丝裂原活化蛋白激酶激酶7(Map2k7)、磷脂酶A2 IVE组(Pla2g4e)、突触小泡蛋白(SYN)和突触后致密蛋白95(PSD95)表达及海马CA1区突触可塑性的影响,探讨EA对糖尿病认知功能减退(DCI)大鼠学习和记忆的改善作用及其机制。方法:采用高糖高脂饲料联合腹腔注射链脲佐菌素(30 mg/kg)诱导2型糖尿病大鼠模型,再通过Morris水迷宫实验筛选出认知减退的大鼠,随机分成DCI组和DCI+EA组,并以正常大鼠作为对照组,每组10只。DCI+EA组在“胰俞”、“内庭”和“后三里”穴处给予针刺治疗,DCI组和对照组只固定于鼠板。造模前后和治疗后,分别检测各组大鼠的随机血糖和逃避潜伏期,EA治疗28 d后,HE染色观察各组大鼠海马CA1区病理变化,透射电镜观察各组大鼠海马CA1区突触数量、突触后致密物(PSD)厚度和突触超微结构变化情况,RT-qPCR和Western blot法检测各组大鼠海马组织Map2k7、Pla2g4e、SYN和PSD95的mRNA和蛋白表达水平。结果:与对照组比较,DCI组大鼠随机血糖水平显著升高(P<0.01),逃避潜伏期显著延长(P<0.01);海马CA1区病理损伤严重,突触超微结构散乱,突触密度显著降低(P<0.01),PSD厚度显著减少(P<0.01);海马组织中Map2k7的mRNA和蛋白表达水平显著升高(P<0.01),Pla2g4e、SYN和PSD95的mRNA和蛋白表达水平均显著降低(P<0.01)。与DCI组比较,DCI+EA组大鼠随机血糖水平显著降低(P<0.01),逃避潜伏期显著缩短(P<0.01);海马CA1区病理损伤减轻,突触超微结构改善明显,突触密度显著升高(P<0.05),PSD厚度增加,但差异无统计学意义(P>0.05);海马组织中Map2k7的mRNA和蛋白表达水平显著降低(P<0.01),Pla2g4e和SYN的mRNA和蛋白表达水平显著升高(P<0.05或P<0.01),PSD95的mRNA表达水平显著升高(P<0.01),PSD95蛋白表达水平升高,但差异无统计学意义(P>0.05)。结论:EA可以改善DCI大鼠的学习和记忆,其机制可能与调节海马Map2k7、Pla2g4e�
AIM:To investigate the effects of electroacupuncture(EA)on learning and memory of diabetic rats with cognitive impairment,and to explore its mechanism.METHODS:A rat model of type 2 diabetes mellitus was established with high-sugar and high-fat diet combined with intraperitoneal injection of streptozotocin(30 mg/kg),and then cognition-impaired rats were screened out by the Morris water maze and randomized into diabetic cognitive impairment(DCI)group and DCI+EA group,with 10 rats in each group.Ten normal rats served as control group.The acupoints of"Hou San Li","Nei Ting"and"Yi Shu"in the rats of DCI+EA group were needled,while the rats in DCI and control groups were merely immobilized on the rat plates.Before and after modeling as well as treatment,random blood glucose level and escape latency of the rats were detected.After 28 d of EA treatment,the pathological changes of rat hippocampal CA1 region were observed by HE staining,and the synaptic number,postsynaptic density(PSD)thickness and synaptic ultrastructure of hippocampal CA1 region were observed under transmission electron microscope.The mRNA and protein levels of mitogen-activated protein kinase kinase 7(Map2k7),phospholipase A2 group IVE(Pla2g4e),synaptophysin(SYN)and postsynaptic density protein 95(PSD95)were detected by RT-qPCR and Western blot.RESULTS:Compared with control group,the rats in DCI group showed significantly higher random blood glucose level(P<0.01),significantly longer escape latency(P<0.01),and severe pathological impairment in the hippocampal CA1 region.The synaptic ultrastructure of the hippocampal CA1 region was scattered,and both synaptic density and PSD thickness were significantly reduced(P<0.01).The hippocampal Map2k7 mRNA and protein levels were significantly increased(P<0.01),while the Pla2g4e,SYN and PSD95 mRNA and protein levels were significantly reduced(P<0.01).Compared with DCI group,the rats in DCI+EA group had significantly lower random blood glucose level(P<0.01),significantly shorter escape latency(P<0.01).The pathologi
作者
付勤
袁爱红
杨骏
樊吟秋
叶敏
张乐乐
钱俊
FU Qin;YUAN Aihong;YANG Jun;FAN Yinqiu;YE Min;ZHANG Lele;QIANJun(The First Clinical College of Anhui University of Chinese Medicine,Hefei 230038,China;The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,China;Anhui Province Key Laboratory of Meridian and Viscera Correlationship,Hefei 230038,China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2024年第4期619-626,共8页
Chinese Journal of Pathophysiology
基金
安徽省自然科学基金资助项目(No.2108085MH308)
袁爱红名医工作室(皖中医药发展秘〔2023〕23号)
第五批全国中医临床优秀人才研修项目(国中医药人教函〔2022〕1号)。
关键词
糖尿病认知功能减退
电针
突触可塑性
学习
记忆
diabetic cognitive impairment
electroacupuncture
synaptic plasticity
learning
memory
