摘要
The vagus nerve(VN)plays an important role in regulating physiological conditions in the gastrointestinal(GI)tract by communicating via the parasympathetic pathway to the enteric nervous system(ENS).However,the lack of knowledge in the neurophysiology of the VN and GI tract limits the development of advanced treatments for autonomic dysfunctions related to the VN.To better understand the complicated underlying mechanisms of the VN-GI tract neurophysiology,it is necessary to use an advanced device enabled by microfabrication technologies.Among several candidates including intraneural probe array and extraneural cuff electrodes,microchannel electrode array devices can be used to interface with smaller numbers of nerve fibers by securing them in the separate channel structures.Previous microchannel electrode array devices to interface teased nerve structures are relatively bulky with thickness around 200µm.The thick design can potentially harm the delicate tissue structures,including the nerve itself.In this paper,we present a flexible thin film based microchannel electrode array device(thickness:11.5µm)that can interface with one of the subdiaphragmatic nerve branches of the VN in a rat.We demonstrated recording evoked compound action potentials(ECAP)from a transected nerve ending that has multiple nerve fibers.Moreover,our analysis confirmed that the signals are from C-fibers that are critical in regulating autonomic neurophysiology in the GI tract.
基金
supported by NIH SPARC Program(OT2 OD028183)
supported in part by the National Science Foundation(United States)under the grant ECCS-1944480。
