摘要
目的:观察龟甲胶对绝经后骨质疏松(PMOP)大鼠能量代谢和骨代谢稳态的影响,及对腺苷酸激活蛋白激酶(AMPK)/沉默信息调节因子2相关酶1(SIRT1)通路的调控作用。方法:将60只雌性SD大鼠随机分为假手术组、模型组、阳性对照组、龟甲胶低、中、高剂量组,每组各10只。除假手术组外,其余大鼠采用双侧卵巢摘除法复制绝经后骨质疏松大鼠模型,以尼尔雌醇片作为阳性对照药,于造模完成后给予尼尔雌醇片1 mg/kg灌胃,1次/d,龟甲胶低、中、高剂量组分别给予0.45,0.90,1.80 g/kg灌胃,2次/d,持续治疗8周。称量大鼠子宫质量并计算子宫指数,双能X线骨密度仪测定L_(4/5)及双侧股骨骨密度,显微CT分析骨形态计量学参数,ELISA法实验检测血清骨钙素(BGP)、骨碱性磷酸酶(BALP)、抗酒石酸酸性磷酸酶5b(TRACP-5b)、Ⅰ型胶原交联羧基末端肽(CTX-Ⅰ)含量,Western Blot法及RT-PCR检测骨组织AMPK/SIRT1通路相关蛋白和mRNA表达。结果:与假手术组相比,模型组子宫指数、L_(4/5)及双侧股骨骨密度明显降低,差异有统计学意义(P<0.05);骨形态计量学参数骨体积分数(BV/TV)、骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th)明显降低,骨小梁间隔(Tb.Sp)明显增高,差异有统计学意义(P<0.05);血清中血清骨钙素、骨碱性磷酸酶、TRACP-5b、CTX-Ⅰ的水平均明显升高,差异有统计学意义(P<0.05);骨组织中p-AMPK/AMPK、SIRT1、PGC1-α的蛋白及AMPK、SIRT1、PGC1-α的mRNA水平明显降低,差异有统计学意义(P<0.05)。龟甲胶高、中、低剂量组治疗后,子宫指数、L_(4/5)及双侧股骨骨密度较模型组明显增高,差异有统计学意义(P<0.05);BV/TV、Tb.N、Tb.Th明显升高,Tb.Sp明显降低,差异有统计学意义(P<0.05);血清中血清骨钙素、骨碱性磷酸酶、TRACP-5b、CTX-Ⅰ的水平均明显降低,差异有统计学意义(P<0.05);同时明显上调了骨组织中p-AMPK/AMPK、SIRT1、PGC1-α的蛋白及AMPK、SIRT1、PGC1-α的mRN
Objective:To observe the efficacy of tortoise shell glue on energy metabolism and bone metabolism homeostasis in postmenopausal osteoporosis(PMOP)rats,as well as its regulatory efficacy on the AMPK/SIRT1 pathway.Methods:60 female SD rats were randomly divided into a sham surgery group,a model group,a positive control group,a high,medium and low dose group of tortoise shell glue,with 10 rats in each group.In addition to the sham surgery group,the other rats used bilateral ovariectomy to duplicate the PMOP model.Nilestriol tablets were used as a positive control drug,1 mg/kg of nilestriol tablets were administered by gavage once a day after the completion of modeling.The low,medium,and high dose groups of tortoise shell glue were given 0.45,0.90,1.80 g/kg by gavage twice a day for 8 weeks.The weight of the rat uterus was weighed and the uterine index was calculated.Dual energy X-ray bone mineral density(BMD)instrument was used to measure the BMD of L_(4/5) and bilateral femurs.Bone morphometric parameters were analyzed by micro CT.The serum levels of osteocalcin(BGP),bone alkaline phosphatase(BALP),anti tartaric acid phosphatase 5b(TRACP-5b),and typeⅠcollagen cross-linked carboxyl terminal peptide(CTX-Ⅰ)were detected by ELISA.Western Blot and RT-PCR were used to detect the expression of AMPK/SIRT1 pathway related proteins and mRNA in bone tissue.Results:The uterine index,L4/5 and bilateral femoral BMD in the model group were significantly lower compared with the sham surgery group(P<0.05).The bone metrology parameters,bone volume fraction(BV/TV),trabecular number(Tb.N),trabecular thickness(Tb.Th),decreased significantly,and trabecular septa(Tb.Sp)were increased significantly(P<0.05).The levels of BGP,BALP,TRACP-5b and CTX-Ⅰin serum were significantly increased(P<0.05).The protein levels of p-AMPK/AMPK,SIRT1,PGC1-αand the mRNA levels of AMPK,SIRT1,PGC1-αin bone tissue were significant reduced(P<0.05).The uterine index,L4/5 and bilateral femoral BMD were significantly increased compared to the model group aft
作者
万文渊
罗娟
彭真灵
朱莹莹
罗杰
WAN Wenyuan;LUO Juan;PENG Zhenling;ZHU Yingying;LUO Jie(Zhuzhou Traditional Chinese Medicine Traumatology Hospital,Zhuzhou 412000,Hunan China)
出处
《中国中医骨伤科杂志》
CAS
2024年第4期18-23,共6页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
湖南省中医药管理局株洲市联合中医药科研课题(E2023016)。
关键词
绝经后骨质疏松
龟甲胶
骨密度
骨代谢
postmenopausal osteoporosis
tortoise shell glue
bone mineral density
bone metabolism
