摘要
目的:观察中药复方乳凝胶对兔耳痤疮模型的药效作用,并初步揭示其作用机制。方法:利用油酸制备兔耳痤疮模型,确认造模成功后开始给药干预,通过病理组织切片HE染色考察复方乳凝胶的治疗效果;利用酶联免疫吸附法(ELISA)测定相关炎症因子,包括肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的表达量;利用免疫组化探究介导痤疮发病的关键信号通路[核转录因子κB(NF-κB)、白细胞分化抗原(CD36)受体、NOD样受体家族3(NLRP3)炎症小体]的表达水平。结果:HE组织染色及免疫组化结果表明复方乳凝胶治疗后兔耳痤疮病变组织得到显著改善,复方乳凝胶组血清中调控病变的炎症因子TNF-α、IL-1β、IL-6和IL-8表达量显著降低(P<0.05或P<0.01);且可介导痤疮发病的关键信号通路NF-κB,下调CD36受体、NLRP3炎症小体的表达水平(P<0.05或P<0.01)。结论:中药复方乳凝胶通过抑制痤疮丙酸杆菌,使与痤疮相关的各种细胞上的TLRs受体及CD36受体表达下调,阻断了NF-κB通路,抑制了NLRP3炎症小体的活化表达,降低了血清中TNF-α、IL-6、IL-8及IL-1β炎症因子的表达水平,从而达到治疗痤疮的效果。
Objective:To investigate the pharmacodynamic action of compound traditional Chinese medicine(TCM)emulsion gel on a rabbit ear acne model and its preliminary mechanism of action.Methods:Oleic acid was used to establish a rabbit ear acne model,and drug intervention was performed after successful modeling.HE staining of histopathological sections was performed to analyze the therapeutic effect of compound emulsion gel;ELISA was used to measure related inflammatory factors,including tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),and interleukin-8(IL-8);immunohistochemistry was used to measure the expression levels of the key signaling pathways mediating the development of acne,including nuclear factor-kappa B(NF-κB),leukocyte differentiation antigen(CD36)receptor,and NOD-like receptor protein 3(NLRP3)inflammasome.Results:HE staining and immunohistochemistry showed that there was a significant improvement in rabbit ear acne lesions after treatment with compound emulsion gel,and the compound emulsion gel group had significant reductions in the expression levels of the inflammatory factors TNF-α,IL-1β,IL-6,and IL-8 in serum(P<0.05 or P<0.01).Compound emulsion gel could mediate the key signaling pathway NF-κB in the development of acne and downregulated the expression levels of CD36 receptor and NLRP3 inflammasome(P<0.05 or P<0.01).Conclusion:The compound TCM emulsion gel exerts a therapeutic effect on acne by inhibiting Propionibacterium acnes,downregulating the expression of TLRs receptor and CD36 receptor on various types of cells associated with acne,blocking the NF-κB pathway,inhibiting the activation and expression of NLRP3 inflammasome,and reducing the expression levels of the inflammatory factors TNF-α,IL-6,IL-8,and IL-1βin serum.
作者
陈伊萌
惠丹丹
穆阿宁
张艳茹
周家豪
梁引库
CHEN Yimeng;HUI Dandan;MU Aning;ZHANG Yanru;ZHOU Jiahao;LIANG Yinku(College of Biological Sciences and Engineering,Shaanxi University of Technology,Hanzhong 723000,Shaanxi,China;Shaanxi Key Laboratory of Bi-resources,Hanzhong 723000,Shaanxi,China;Collaborative Innovation Center of Comprehensive Development of Biological Resources in Qinling-Daba Mountains of Southern Shaanxi,Hanzhong 723000,Shaanxi,China)
出处
《湖南中医杂志》
2024年第3期153-159,共7页
Hunan Journal of Traditional Chinese Medicine
基金
陕西理工大学重点科研机构开放课题研究基金项目(SLGPT2019KF04-05)
陕西省科技计划项目(2023-CXPT-24,2023-YBSF-017)。
关键词
中药复方乳凝胶
痤疮
疗效评价
作用机制
炎症因子
compound traditional Chinese medicine emulsion gel
acne
therapeutic effect
mechanism of action
inflammatory factor
