摘要
为明确外源褪黑素对干旱胁迫下旺长期烤烟生理特性的影响,采用盆栽试验,以西南烟区主栽品种K326为材料,研究了根部浇灌和叶面喷施不同浓度(0、0.05、0.10、0.15、0.20、0.40 mmol/L)褪黑素对干旱胁迫下旺长期烤烟的生理调控效应,并利用主成分分析筛选出缓解烤烟干旱胁迫的指标,同时通过隶属函数法进行综合评价。结果表明,干旱胁迫可导致烤烟生物量降低,生长受到明显抑制。外源褪黑素能有效提高干旱胁迫下烤烟生物量,提高叶片相对含水率(RWC)、叶绿素含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、脯氨酸(Pro)和可溶性蛋白(SP)含量,降低叶片丙二醛(MDA)含量和超氧阴离子(O_(2)^(-))产生速率。主成分分析表明,在褪黑素缓解烤烟干旱胁迫评价的指标中,以抗氧化因子占比最大,其次是光合因子和渗透调节因子。通过隶属函数综合评价分析可知,烟株叶面喷施0.10 mmol/L褪黑素溶液处理效果最佳。因此,施用外源褪黑素可缓解干旱胁迫对旺长期烤烟生长的抑制作用,增强烤烟对干旱胁迫的适应性。
To investigate the influence of exogenous melatonin on the physiological characteristics of flue-cured tobacco at fast-growing stage under drought stress,a pot experiment was conducted using the flue-cured tobacco cultivar K326 in southwestern tobacco growing areas.The physiological regulatory effects of root irrigation and foliar application of melatonin at different concentrations(0,0.05,0.10,0.15,0.20,0.40 mmol/L)on K326 under drought stress were investigated,and the indicators that could alleviate drought stress on K326 were screened using principal component analysis.Meanwhile,a comprehensive evaluation was carried out using the membership function method.The results indicated that drought stress caused a decrease in the biomass of cured tobacco and significantly inhibited plant growth.Exogenous melatonin effectively increased biomass under drought stress,increased relative leaf water content(RWC),chlorophyll content,superoxide dismutase(SOD)activity,peroxidase(POD)activity,catalase(CAT)activity,proline(Pro)and soluble protein(SP)content,while decreasing leaf malondialdehyde(MDA)content and superoxide anion(O_(2)^(-))production rate of the flue-cured tobacco.Principal component analysis revealed that antioxidant factors had the highest proportion among the evaluated indicators for alleviation of drought stress by melatonin,followed by photosynthetic factors and osmotic regulation factors.Comprehensive evaluation using the membership function method showed that foliar application of 0.10 mmol/L melatonin solution gave the best results.Therefore,the application of exogenous melatonin can potentially alleviate the inhibitory effects of drought stress on the growth of flue-cured tobacco at the fast-growing stage and enhance its adaptability to drought conditions.
作者
崔凯东
张雪
顾开元
郭金格
汪芬芬
刘铭
周玉玲
杨奇
谢小玉
CUI Kaidong;ZHANG Xue;GU Kaiyuan;GUO Jinge;WANG Fenfen;LIU Ming;ZHOU Yuling;YANG Qi;XIE Xiaoyu(College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,China)
出处
《烟草科技》
CAS
CSCD
北大核心
2024年第4期42-52,共11页
Tobacco Science & Technology
基金
云南省烟草公司大理州公司科技计划项目“烤冻多用房开发及配套应用模式研究”(DLYC2022006)
重庆市大学生创新训练项目(S202310635219)。
关键词
烤烟
干旱胁迫
褪黑素
生理指标
主成分分析
综合评价
Flue-cured tobacco
Drought stress
Melatonin
Physiological index
Principal component analysis
Comprehensive evaluation
