摘要
目的 探讨胃癌患者组织中微小RNA-597(miR-597)、微小RNA-3188(miR-3188)表达情况,以及与临床病理特征和预后的关系。方法 选取2019年1月至2020年6月在东莞市人民医院住院并行手术治疗的96例胃癌患者为研究对象,收集患者术中切除的胃癌组织标本和癌旁组织标本,同时整理收集患者的临床病理资料。采用实时荧光定量PCR(qPCR)法检测组织中miR-597、miR-3188相对表达水平,分析miR-597、miR-3188表达与患者临床病理特征的关系;采用Kaplan-Meier法分析胃癌组织miR-597、miR-3188表达与患者预后的关系,多因素COX回归分析胃癌患者预后的影响因素。结果 与癌旁组织相比,胃癌组织中miR-597和miR-3188相对表达水平均显著低于癌旁组织(P<0.05)。胃癌组织中miR-597的表达与患者的肿瘤分化程度、TNM分期和淋巴结转移有关(P<0.05),miR-3188的表达与患者的浸润深度、肿瘤分化程度、TNM分期和淋巴结转移有关(P<0.05)。胃癌组织miR-597高表达患者3年生存率高于低表达患者(P<0.05),胃癌组织miR-3188高表达患者3年生存率也高于低表达患者(P<0.05)。肿瘤分化程度、TNM分期和淋巴结转移是胃癌患者死亡的独立危险因素,miR-597和miR-3188是胃癌患者的独立保护因素(P<0.05)。结论 miR-597与miR-3188在胃癌组织中低表达,miR-597与miR-3188可能是评估胃癌患者预后的重要指标。
Objective To investigate the expression of microRNA-597(miR-597)and microRNA-3188(miR-3188)in gastric cancer tissues and their relationship with clinical pathological features and prognosis.Methods From January 2019 to June 2020,a total of 96 gastric cancer patients who were hospitalized for surgical treatment in this hospital were collected as the research subjects.Gastric cancer tissue samples and adjacent tissue samples were collected during the surgery,and clinical and pathological data of the patients were also collected.qPCR method was applied to detect the relative expression levels of miR-597 and miR-3188 in tissues;the relationship between the expression of miR-597 and miR-3188 and the clinical and pathological characteristics of patients was analyzed.Kaplan-Meier method was used to analyze the relationship between the expression of miR-597 and miR-3188 in gastric cancer tissue and patient prognosis.Multivariate COX regression was applied to analyze the prognostic factors of gastric cancer patients.Results The relative expression levels of miR-597 and miR-3188 in gastric cancer tissue were obviously lower than those in adjacent tissues(P<0.05).The expression of miR-597 in gastric cancer tissue was related to the degree of tumor differentiation,TNM staging,and lymph node metastasis of patients(P<0.05).The expression of miR-3188 was related to the depth of infiltration,tumor differentiation,TNM staging,and lymph node metastasis of patients(P<0.05).The 3-year survival rate of patients with high expression of miR-597 in gastric cancer tissue was higher than that of patients with low expression(P<0.05),the 3-year survival rate of patients with high expression of miR-3188 in gastric cancer tissue was also higher than that of patients with low expression(P<0.05).The degree of tumor differentiation,TNM staging,and lymph node metastasis were independent risk factors for death in gastric cancer patients,while miR-597 and miR-3188 were independent protective factors for gastric cancer patients(P<0.05).Conclusion mi
作者
袁志鹏
郑锐年
袁淑卿
陈镜塘
吴依芬
YUAN Zhipeng;ZHENG Ruinian;YUAN Shuqing;CHEN Jingtang;WU Yifen(Guangdong Medical University,Zhangjiang,Guangdong 523000,China;Department of Oncology,Dongguan People′s Hospital,Dongguan Guangdong 523000,China;Department of Ultrasonic,Dongguan Maternal and Child Health Care Hospital,Dongguan,Guangdong 523000,China;Shantou University Medical College,Shantou,Guangdong 515041,China)
出处
《国际检验医学杂志》
CAS
2024年第8期976-980,共5页
International Journal of Laboratory Medicine
