摘要
目的探讨预防性使用非瑟酮(Fisetin)对急性前列腺损伤大鼠的保护作用及机制。方法4~6周龄成年雄性Wistar大鼠通过对大鼠前列腺左、右腹侧叶各注射2针3%角叉菜胶生理盐水溶液,每针50μl,共注射200μl的方法,构建急性前列腺损伤大鼠模型。将36只大鼠采用随机数字表法分为6组(每组6只),假手术(Sham)组、模型组、Fisetin低剂量组(25 mg/kg)、中剂量组(50 mg/kg)、高剂量组(100 mg/kg)、阳性药物组(普乐安片1.08 g/kg),Fisetin治疗组及阳性药物组分别在造模前3 d每天1次,造模当天及造模后3 d每天1次(共7次)进行药物灌胃治疗。Sham组和模型组给予相同剂量的生理盐水,在注射角叉菜胶后3 d后处死大鼠,收集前列腺组织,通过苏木精-伊红(HE)染色分析每组大鼠前列腺组织病理学变化;酶联免疫吸附试验(ELISA)、实时荧光定量(RT-qPCR)、免疫组织化学法(IHC)分析炎性因子表达,蛋白质印迹法(Western blot)检测炎性因子及Toll样受体4(TLR4)、核因子-κB(NF-κB)与磷酸化核因子-κB(p-NF-κB)的蛋白表达水平。多组间比较采用单因素方差分析,两组间比较采用t检验。结果模型组大鼠前列腺组织中IL-6、IL-1β、TNF-α的蛋白表达水平明显高于Sham组、Fisetin高剂量组和阳性药物组[IL-6:(245.10±7.73)pg/ml比(77.65±6.28)、(191.10±10.20)、(197.80±9.31)pg/ml,t=6.632、4.237、3.924,P<0.05;0.90±0.05比0.61±0.05、0.36±0.08、0.31±0.07,t=4.218、5.918、6.684,P<0.05;IL-1β:(55.59±4.06)pg/ml比(19.32±2.68)、(40.32±2.75)、(40.22±2.06)pg/ml,t=3.453、3.126、3.385,P<0.05;1.07±0.08比0.66±0.02、0.46±0.05、0.37±0.06,t=5.253、6.951、7.018,P<0.05;TNF-α:(188.40±14.36)pg/ml比(45.52±7.54)、(125.50±5.92)、(118.60±14.89)pg/ml,t=7.158、4.135、3.372,P<0.05;0.84±0.06比0.53±0.05、0.44±0.04、0.40±0.02,t=4.824、5.827、6.785,P<0.05],差异有统计学意义。模型组大鼠前列腺组织中TLR-4、NF-κB/p-NF-κB水平明显高于Sham组、Fisetin
Objective To investigate the protective effect and mechanism of prophylactic use of Fisetin on acute prostate injury in rats.MethodsA rat model of acute prostate injury was established by injecting 2 needles of 3%sterile carrageenan saline solution into the left and right ventral lobes of the prostate of adult male Wistar rats(purchased from Wuhan Wanqian Jiaxing Biological Technology Co.,Ltd.),each needle of 50μl,a total of 200μl.The rats were randomly divided into 6 groups(6 cases each):sham operation group,model group,Fisetin low dose group(25 mg/kg),Fisetin middle dose group(50 mg/kg),Fisetin high dose group(100 mg/kg),positive drug group(1.08 g/kg).Fisetin treatment group and positive drug group were treated with intragastric administration once a day for 3 days before modeling,once a day on the day of modeling and 3 days after modeling(7 times in total).The sham operation group and the model group were given the same dose of normal saline.The rats were sacrificed 3 days after injection of carrageenan.The prostate tissue was collected and the pathological changes of prostate tissue in each group were analyzed by hematoxylin-eosin(HE)staining.The expression of inflammatory factors was analyzed by enzyme-linked immunosorbent assay(ELISA),real-time fluorescence quantitative PCR(RT-qPCR)and immunohistochemistry.The protein expression levels of inflammatory factors,toll-like receptor 4(TLR4),nuclear factor kappa B(NF-κB)and phosphorylated nuclear factor kappa B(p-NF-κB)were detected by Western blotting.One-way analysis of variance was used for comparison between multiple groups,and t test was used for comparison between two groups.ResultsIn the prostate tissues of rats,IL-6,IL-1βand TNF-αprotein expression levels were significantly higher in the model group than in the Sham group,the Fisetin high-dose group,and the positive drug group[IL-6:(245.10±7.73)vs.(77.65±6.28),(191.10±10.20),(197.80±9.31)pg/ml,t=6.632,4.237,3.924,P<0.05;0.90±0.05 vs.0.61±0.05,0.36±0.08,0.31±0.07,t=4.218,5.918,6.684,P<0.05;IL
作者
司鹏辉
曹远飞
白灿
刘同族
Si Penghui;Cao Yuanfei;Bai Can;Liu Tongzu(Department of Urology,Zhongnan Hospital,Wuhan University,I Wuhan 430071,China)
出处
《中华实验外科杂志》
CAS
2024年第3期541-544,共4页
Chinese Journal of Experimental Surgery
