摘要
目的:观察电针对高脂饮食诱导的肥胖小鼠的治疗作用及对肥胖小鼠肠道菌群的影响。方法:从50只C57BL/6J小鼠中随机筛选12只为空白组,予以正常饮食,剩余38只小鼠予以高脂饮食饲养16周后,将造模成功的小鼠随机分为模型组和电针组。治疗4周后,对小鼠的体质量、脂肪重量、血脂水平及病理改变等进行评价,观察电针对肥胖小鼠的治疗效果。并通过16S rDNA高通测序技术分析小鼠微生物的变化,分析电针治疗肥胖小鼠的作用机制。结果:与模型组比较,电针组小鼠体质量、脂肪重量及TC、TG、LDL显著下降(P<0.01),TG降低(P<0.05),电针明显改善肥胖小鼠的病理损伤情况。肠道菌群检测显示,电针干预后,小鼠的肠道菌群数量明显回升,高脂饮食饲养小鼠肠道菌群中的厚壁菌门丰度,提高拟杆菌门丰度下降,Muribaculaceae_norank占比明显上升(30.74%)。结论:电针对肥胖小鼠有良好的治疗效果,能明显降低肥胖小鼠体质量、脂肪重量与血脂水平,改善肥胖小鼠的病理损伤情况,具有调控肠道菌群的作用。
Objective:To observe the therapeutic effect of electro-acupuncture(EA) on high-fat diet induced obese mice and to study its impact on intestinal flora in obese mice.Methods:12 mice were randomly selected as the blank control from 50 C57BL/6J mice,and were fed with normal diet;whereas the remaining 38 mice were fed with high-fat diet for 16 weeks.The successful modeling mice were randomly divided into the model group and the EA group.After 4 weeks of the treatment,body mass,fat weight,blood lipid levels and pathological changes of mice were evaluated to observe the therapeutic effect of EA on obese mice.The changes in mice microbiota were analyzed by using 16S rDNA high-throughput sequencing technology to explore the action mechanism of EA in treating obese mice.Results:Compared with those in the model group,the body mass,fat weight,TC,TG and LDL were significantly decreased in the EA group(P<0.01,P<0.05).EA significantly improved the pathological damages of obese mice.The detection of intestinal flora showed that after EA intervention,the number of gut microbiota in mice significantly increased.The abundance of Firmicutes in the gut microbiota of mice fed on a high-fat diet increased,whereas the abundance of Bacteroidetes decreased,and the proportion of Muribaculaceae_norank significantly increased(accounted for 30.74%).Conclusion:EA has a good therapeutic effect on obese mice,it can significantly reduce body mass,fat weight and blood lipid levels,improve pathological damages,and regulate intestinal flora in obese mice.
作者
秦鸿宇
杨添淞
巴特
孙忠人
冯楚文
张淼
王玉琳
QIN Hongyu;YANG Tiansong;BA Te;SUN Zhongren;FENG Chuwen;ZHANG Miao;WANG Yulin(Heilongjiang University of Chinese Medicine,Harbin 150040,China;The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150001,China)
出处
《针灸临床杂志》
2024年第3期78-84,共7页
Journal of Clinical Acupuncture and Moxibustion
基金
国家自然科学基金面上项目,编号:82074539
黑龙江省自然科学基金,编号:LH2020H092
教育部“春晖计划”合作科研项目,编号:HZKY20220308-202201357
黑龙江卫生健康委课题,编号:20222121021106。
关键词
电针
高脂饮食
肥胖
小鼠
肠道菌群
Electro-acupuncture
High fat diet
Obese
Mice
Intestinal flora
